Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 12F10] (IgG2b) to Mouse TNNI / Troponin I Skeletal
IHC - Paraffin, Western blot
Mouse TNNI / Troponin I Skeletal
Mouse, Human (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [12F10]
Protein A purified
IHC - Paraffin (10 µg/ml)
Specificity and Use
Mouse Skeletal muscle Troponin I.
Cross-reactivity with cardiac TNI is less than 0.05%.
Immunohistochemistry: LS-B4037 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4037 was determined to be 10 ug/ml.
Anti-TNNI / Troponin I Skeletal antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4037 concentration 10 ug/ml.
Requested From: United States
Date Requested: 10/28/2016