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Anti-TNFSF14 / LIGHT Antibody (Biotin) LS-C104600

Note: This antibody replaces LS-C54175, LS-C37192

Ordering

Wt. Vol. Conc. Price
25 µg - - $285
50 µg - - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular TNFSF14 / LIGHT Antibodies

Anti-TNFSF14 / LIGHT Antibody LS-C104596
Rabbit Polyclonal to Human TNFSF14 / LIGHT
Human
Western blot, ELISA
Unconjugated
Western blot Image
Anti-TNFSF14 / LIGHT Antibody (clone 4E3) LS-C133566
Mouse Monoclonal [clone 4E3] (IgG2a,k) to Human TNFSF14 / LIGHT
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-TNFSF14 / LIGHT Antibody (clone Chris-2) LS-C343730
Mouse Monoclonal [clone Chris-2] (IgG2b) to Human TNFSF14 / LIGHT
Human
Western blot, Flow Cytometry, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human TNFSF14 / LIGHT
Human
Western blot, ELISA (applications tested for the base form of this product only)
Biotin Conjugated

Details

Human TNFSF14 / LIGHT
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Biotin. Also available Unconjugated.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

TNFSF14 / LIGHT antibody was raised against e.coli derived recombinant Human LIGHT.
Human LIGHT
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hLIGHT by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hLIGHT. To detect hLIGHT by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human LIGHT (LS-C104596) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hLIGHT. Western Blot: To detect hLIGHT by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLIGHT is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
Sterile PBS containing 0.1% BSA
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About TNFSF14 / LIGHT

O43557 NM_003807 NP_003798.2

TNFSF14 Antibody, CD258 antigen Antibody, CD258 Antibody, Delta transmembrane LIGHT Antibody, Herpesvirus entry mediator A Antibody, HVEM-L Antibody, TR2 Antibody, HVEML Antibody, LIGHT Antibody, LTg Antibody

TNFSF14 / LIGHT is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF14, which is a member of the tumor necrosis factor receptor superfamily, and which is also known as a herpesvirus entry mediator (HVEM). This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus.

Western blot

Western blot
Western Blot (reducing) of TNFSF14 antibody LS-C104600 This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western Blot (non-reducing) of TNFSF14 antibody LS-C104600 This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western Blot (reducing) of TNFSF14 antibody LS-C104596. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western Blot (non-reducing) of TNFSF14 antibody LS-C104596. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of TNFSF14 antibody LS-C104596. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/9/2016

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