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Antibodies > TNFSF14 / LIGHT > LS-C108144
 
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Catalog Number Size Price
LS-C108144-100 100 µg (0.5 mg/ml) $503 
TNFSF14 / LIGHT Antibody - TNFSF14 / LIGHT antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
TNFSF14 / LIGHT Antibody - TNFSF14 antibody (1 ug/ml) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TNFSF14 / LIGHT Antibody - TNFSF14 / LIGHT antibody (0.03µg/ml) staining of Human Cerebellum (A) and Frontal Cortex (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
TNFSF14 / LIGHT Antibody - TNFSF14 / LIGHT antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
TNFSF14 / LIGHT Antibody - TNFSF14 antibody (1 ug/ml) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TNFSF14 / LIGHT Antibody - TNFSF14 / LIGHT antibody (0.03µg/ml) staining of Human Cerebellum (A) and Frontal Cortex (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
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Polyclonal Goat anti‑Human TNFSF14 / LIGHT Antibody (aa90‑103, IF, WB) LS‑C108144

Polyclonal Goat anti‑Human TNFSF14 / LIGHT Antibody (aa90‑103, IF, WB) LS‑C108144

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Antibody:
TNFSF14 / LIGHT Goat anti-Human Polyclonal (aa90-103) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$503
LS-C108144-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
TNFSF14 / LIGHT Goat anti-Human Polyclonal (aa90-103) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
LIGHT antibody LS-C108144 is an unconjugated goat polyclonal antibody to human LIGHT (TNFSF14) (aa90-103). Validated for IF, Peptide-ELISA and WB.
Target
Human TNFSF14 / LIGHT
Synonyms
TNFSF14 | CD258 antigen | CD258 | Delta transmembrane LIGHT | Herpesvirus entry mediator A | HVEM-L | TR2 | HVEML | LIGHT | LTg
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-HEVNPAAHLTGANS, from the internal region of the protein sequence according to NP_003798.2NP_742011.1.
Epitope
aa90-103
Specificity
Human TNFSF14 / LIGHT. This antibody is expected to recognize both reported isoforms (NP_003798.2; NP_742011.1).
Applications
  • Immunofluorescence (10 µg/ml)
  • Western blot (0.03 - 0.1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Usage
Western Blot: Approx 22kD band observed in Human Brain (Cerebellum and Frontal Cortex) lysates (calculated MW of 22.4kD according to NP_742011.1). Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TNFSF14 / LIGHT
O43557 NM_003807 NP_003798.2

Publications (0)

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Featured Products

TNFSF14 / LIGHT Antibody - Western Blot (reducing) of TNFSF14 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
TNFSF14 / LIGHT Antibody LS‑C104596
Species: Human
Applications: Western blot, ELISA
TNFSF14 / LIGHT Antibody - Immunoperoxidase of monoclonal antibody to TNFSF14 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml]
TNFSF14 / LIGHT Antibody (clone 4E3) LS‑C133566
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
TNFSF14 / LIGHT Antibody - Western Blot (reducing) of TNFSF14 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
TNFSF14 / LIGHT Antibody (Biotin) LS‑C104600
Species: Human
Applications: Western blot, ELISA
TNFSF14 / LIGHT Antibody - Staining of PMA and ionomycin-stimulated normal human peripheral blood cells with APC anti-human CD8a (RPA-T8) (LS-C107300) and 0.25 ug of PE Mouse IgG1, K isotype control (left) or PE anti-human CD258 (7-3) (right). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
TNFSF14 / LIGHT Antibody (clone 7‑3 (7), PE) LS‑C105829
Species: Human
Applications: Flow Cytometry
TNFSF14 / LIGHT Antibody - Immunofluorescence analysis of HeLa cells, using TNF14 Antibody. The picture on the right is blocked with the synthesized peptide.
TNFSF14 / LIGHT Antibody (aa51‑100) LS‑C118682
Species: Human, Mouse
Applications: Immunofluorescence, Peptide Enzyme-Linked Immunosorbent Assay
TNFSF14 / LIGHT Antibody - Western blot of recombinant TNFSF14 / LIGHT. This image was taken for the unconjugated form of this product. Other forms have not been tested.
TNFSF14 / LIGHT Antibody (aa51‑239, Biotin) LS‑C300642
Species: Mouse
Applications: Western blot
See all TNFSF14 / LIGHT Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/23/2024
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