LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All ELISA Kits
  • Traditional ELISA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Phospho-Specific ELISA Kits
  • ELISA Development Kits
  • Chemiluminescent CLIA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Protocols
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-TNFSF11 / RANKL / TRANCE Antibody (Internal) IHC-plus™ LS-B1425

Note: This antibody replaces LS-C19647, LS-C38730

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml Unavailable

Most Popular TNFSF11 / RANKL / TRANCE Antibodies

Anti-TNFSF11 / RANKL / TRANCE Antibody (aa1-317, Azide-free) LS-C19335
Mouse Monoclonal (IgG1,k) to Mouse TNFSF11 / RANKL / TRANCE
Mouse, Human
IHC - Paraffin, Western blot
Unconjugated, Azide-free
Western blot Image
Anti-TNFSF11 / RANKL / TRANCE Antibody (Internal) IHC-plus™ LS-B1425
Rabbit Polyclonal to Human TNFSF11 / RANKL / TRANCE
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-TNFSF11 / RANKL / TRANCE Antibody (Biotin) LS-C86684
Goat Polyclonal to Human TNFSF11 / RANKL / TRANCE
Human
Western blot, ELISA
Biotin Conjugated
Anti-TNFSF11 / RANKL / TRANCE Antibody LS-C104835
Mouse Monoclonal (IgG1) to Human TNFSF11 / RANKL / TRANCE
Human
ELISA
Unconjugated
Enzyme-Linked Immunosorbent Assay Image
Anti-TNFSF11 / RANKL / TRANCE Antibody (aa64-245, clone TS106) LS-C191486
Mouse Monoclonal [clone TS106] (IgG3) to Human TNFSF11 / RANKL / TRANCE
Human
Western blot
Unconjugated

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal to Human TNFSF11 / RANKL / TRANCE
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated

Details

Human TNFSF11 / RANKL / TRANCE
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.25 - 0.5 µg/ml)

Specificity and Use

TNFSF11 / RANKL / TRANCE antibody was raised against synthetic peptide from human TNFSF11 / RANKL.
LS-E30281 - Liquid - 50 µg 0.2 mg/ml - $145.00
Immunizing peptide used to generate LS-B1425. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Internal
14 amino acid peptide from near the center of human sRANK-L
Immunohistochemistry: LS-B1425 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1425 was determined to be 5 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

Publications (1)

1
Transient gamma-secretase inhibition accelerates and enhances fracture repair likely via Notch signaling modulation. Wang C, Shen J, Yukata K, Inzana JA, O'Keefe RJ, Awad HA, Hilton MJ. Bone. Apr;73:77-89. [Full Text Article] [PubMed:25527421] [PMC:PMC4336841]

About TNFSF11 / RANKL / TRANCE

O14788 AF053712 AAC39731.1

TNFSF11 Antibody, CD254 Antibody, CD254 antigen Antibody, HRANKL2 Antibody, OPTB2 Antibody, TRANCE Antibody, ODF Antibody, OPGL Antibody, SOdf Antibody, Osteoprotegerin ligand Antibody, RANKL Antibody

TNFSF11 / RANKL / TRANCE is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss.

Immunohistochemistry

Immunohistochemistry
Anti-RANKL antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1425 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-RANKL antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1425 concentration 5 ug/ml.

Western blot

Western blot
Western blot of sRANK-L in rat liver tissue lysate with sRANK-L antibody at (A) 0.25 and (B) 0.5 ug/ml.

Requested From: 
Date Requested: 4/24/2017

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2017 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number