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Anti-TNFSF11 / RANKL / TRANCE Antibody (aa72-316) LS-C150261

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular TNFSF11 / RANKL / TRANCE Antibodies

Anti-TNFSF11 / RANKL / TRANCE Antibody (Internal) IHC-plus™ LS-B1425
Rabbit Polyclonal to Human TNFSF11 / RANKL / TRANCE
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-TNFSF11 / RANKL / TRANCE Antibody (Biotin) LS-C86684
Goat Polyclonal to Human TNFSF11 / RANKL / TRANCE
Human
Western blot, ELISA
Biotin Conjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Mouse TNFSF11 / RANKL / TRANCE
Mouse
IHC - Frozen, Western blot, ELISA, Neutralization
Unconjugated

Details

Mouse TNFSF11 / RANKL / TRANCE
Goat
Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Frozen (5 - 15 µg/ml)
  • Western blot (0.1 µg/ml)
  • ELISA
  • Neutralization

Specificity and Use

TNFSF11 / RANKL / TRANCE antibody was raised against mouse myeloma cell line NS0-derived recombinant mouse TRANCE/TNFSF11/RANK L Arg72-Asp316 Accession # O35235.
aa72-316
Detects mouse TRANCE/TNFSF11/RANK L in ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant.
Western Blot, 0.1 µg/ml, Recombinant Mouse TRANCE/TNFSF11/RANK L. Immunohistochemistry, 5-15 µg/ml, Perfusion fixed frozen sections of mouse thymus. Mouse TRANCE/TNFSF11/RANK L Sandwich Immunoassay, Reagent. ELISA Capture, 0.2-0.8 µg/ml, Mouse TRANCE/TNFSF11/RANK L Antibody. ELISA Detection, 0.1-0.4 µg/ml, Mouse TRANCE/TNFSF11/RANK L Biotinylated Antibody. Standard, Recombinant Mouse TRANCE/TNFSF11/RANK L. Neutralization, Measured by its ability to neutralize TRANCE/TNFSF11/RANK L-induced osteoclast-like cell formation in mouse. splenocytes. The Neutralization Dose (ND50) is typically 0.02-0.06 µg/ml in the presence of 30 ng/ml Recombinant Mouse TRANCE/TNFSF11/RANK L.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About TNFSF11 / RANKL / TRANCE

O14788 AF053712 AAC39731.1

TNFSF11 Antibody, CD254 Antibody, CD254 antigen Antibody, HRANKL2 Antibody, OPTB2 Antibody, TRANCE Antibody, ODF Antibody, OPGL Antibody, SOdf Antibody, Osteoprotegerin ligand Antibody, RANKL Antibody

TNFSF11 / RANKL / TRANCE is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss.

Neutralization

Neutralization
Osteoclast-like Cell Formation Induced by TRANCE/ TNFSF11/RANK L and Neutralization by Mouse TRANCE/TNFSF11/RANK L Antibody. In the presence of Recombinant Mouse M-CSF (20 ng/ml), Recombinant Mouse TRANCE/TNFSF1 1/RANK L induces osteoclast-like cell formation in mouse splenocytes in a dose-dependent manner (orange line), as measured by TRAP (tartrate-resistant acid phosphatase) solution assay. Under these conditions, osteoclast-like cell formation elicited by Recombinant Mouse TRANCE/TNFSF1 1/ RANK L (30 ng/ml) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TRANCE/TNFSF1 1/RANK L Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.02-0.06 ug/ml.
 

Requested From: United States
Date Requested: 12/6/2016

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