Human TNFSF10 / TRAIL

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated

Protein A purified

Unmodified

E.coli derived recombinant Human TRAIL/Apo2L.

Human TRAIL/Apo2L

• Western blot (0.25 - 0.5 µg/ml)
• ELISA (2 - 4 µg/ml)

Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human TRAIL/Apo2L (10 ng/ml), a concentration of 0.30-0.60 ug/ml of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect at least 500 pg/ml of recombinant human sTRAIL/APO2L when used with biotinylated antigen affinity purified anti-human sTRAIL/APO2L (LS-C104497) as the detection antibody at a concentration of approximately 1-2 ug/ml. Western Blot: To detect Human sTRAIL/Apo2L by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human sTRAIL/Apo2L is 0.25-1 ng/lane, under reducing or non-reducing conditions.

Lyophilized from PBS

Reconstitute in sterile water to 1 mg/ml.

Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P50591 NCBI: NM_003810 NP_003801.1