Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC, IHC - Paraffin, Western blot, ELISA, Neutralization
Mouse Monoclonal (IgG1) to Human TNFRSF1B / TNFR2
IHC, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Human TNFRSF1B / TNFR2
Human, Monkey (tested or 100% immunogen sequence identity)
IHC - Frozen
Western blot (1:10)
Flow Cytometry (1:10)
Specificity and Use
Recognizes the extracellular part of the human TNF-RII. It also reacts with the soluble receptor. Species cross-reactivity: rhesus and cynomolgus natural TNF-RII.
Suitable for use in Flow Cytometry, Immunohistochemistry (frozen), Immunoprecipitation, Western Blot and ELISA. Immunohistology: 1:10. Flow Cytometry: 1:10. Western Blot: 1:10. Positive control: Immunohistochemistry: Human lymph nodes.
PBS, 0.02% sodium azide, 0.1% BSA.
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.