Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hsTNF-receptor (0.3 ug/ml), a concentration of 0.9-1.1 ug/ml of this antibody is required. ELISA: To detect hsTNF-receptor by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human sTNF-receptor (LS-C104403
) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hsTNF-receptor. Western Blot: To detect hsTNF-receptor by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTNF-receptor is 1.5-3 ng/lane, under either reducing or non-reducing conditions.