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Anti-TNFRSF12A / TWEAK Receptor Antibody LS-C150267


Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular TNFRSF12A / TWEAK Receptor Antibodies

Anti-TNFRSF12A / TWEAK Receptor Antibody (clone ITEM-4) LS-C105966
Mouse Monoclonal [clone ITEM-4] (IgG2b,k) to Human TNFRSF12A / TWEAK Receptor
IHC - Frozen, Western blot, Flow Cytometry
Flow Cytometry Image
Anti-TNFRSF12A / TWEAK Receptor Antibody LS-C150061
Goat Polyclonal (IgG) to Human TNFRSF12A / TWEAK Receptor
IHC, Western blot, ELISA, Neutralization

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Mouse TNFRSF12A / TWEAK Receptor
IHC, Western blot, Flow Cytometry, ELISA


Mouse TNFRSF12A / TWEAK Receptor
Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified


  • IHC
  • Western blot (0.1 - 0.2 µg/ml)
  • Flow Cytometry (50 µg/ml)
  • ELISA (0.8 µg/ml)

Specificity and Use

TNFRSF12A / TWEAK Receptor antibody was raised against purified, E. coli-derived, recombinant mouse TNF-related Weak inducer of Apoptosis Receptor (rmTWEAK R) extracellular domain.
Mouse TWEAK R extracellular domain.
Western blot-This antibody can be used at 0.1-0.2 µg/ml with the appropriate secondary reagents to detect mouse TWEAK R. The detection limit for rmTWEAK R is approximately 2 ng/lane under non-reducing and reducing conditions. Immunohistochemistry-This antibody will detect TWEAK R in cells and tissues. The working dilution is 5-15 µg/ml. Flow Cytometry-This antibody has been tested on MS-1 cells for use in flow cytometry. Dilute this antibody to 50 µg/ml and add 10 ul of the diluted solution to 1-2.5 x 105 cells in a total reaction volume not exceeding 200 ul. The binding of. unlabeled antibodies may be visualized by adding 10 ul of a 25 µg/ml stock solution of a secondary developing reagent such as anti-goat IgG conjugated to a fluorochrome. ELISA Capture-This antibody can be used as a capture antibody in a mouse TWEAK R ELISA in combination with biotinylated, mouse TWEAK R affinity purified polyclonal detection antibody. A general protocol is provided on the next page. Using plates coated with 100 ul/well of the detection antibody, in combination with 100 ul/well of the capture antibody at 0.8 µg/ml, an ELISA for. sample volumes of 100 ul can be obtained. To arrive at the optimal dose range for this ELISA, set up a two-fold dilution series of the protein standard starting with 20 ng/ml. In this format, this antibody shows less than 2% cross-reactivity with rhTWEAKR.


Lyophilized from 0.2 µm filtered PBS, pH 7.4, 5% trehalose
Reconstitute with 200 ul sterile PBS.
Store at -20 to -70 C. Stable for 12 months from date of receipt.
For research use only.

About TNFRSF12A / TWEAK Receptor

Q9NP84 NM_016639 NP_057723.1

TNFRSF12A Antibody, CD266 antigen Antibody, CD266 Antibody, FGF-inducible 14 Antibody, FN14 Antibody, Tweak-receptor Antibody, TWEAKR Antibody

Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins.

Requested From: United States
Date Requested: 10/21/2016

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