Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
TNFRSF11A / RANK is a member of the TNF-receptor superfamily. This receptors can interact with various TRAF family proteins, through which this receptor induces the activation of NF-kappa B and MAPK8/JNK. This receptor and its ligand are important regulators of the interaction between T cells and dendritic cells. This receptor is also an essential mediator for osteoclast and lymph node development.
Detection of RANK in RAW cells. Lane 1. Western blot detection of RANK in RAW, a mouse cell line. Lane 2 & 3. IP/Western blot of RANK. RANK protein from RAW cell lysate was immunoprecipitated either with control antibody (lane 2) or IMG-325 (lane 3), the immunoprecipitated proteins were resolved on SDS-PAGE. RANK was detected by Western blot of IMG-325 antibody.
Flow cytometry analysis of RANK using antibody at 5 ug/10^6 RAW cells.