Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
TNFRSF11A / RANK is a member of the TNF-receptor superfamily. This receptors can interact with various TRAF family proteins, through which this receptor induces the activation of NF-kappa B and MAPK8/JNK. This receptor and its ligand are important regulators of the interaction between T cells and dendritic cells. This receptor is also an essential mediator for osteoclast and lymph node development.
RANK/TNFRSF11A in Rat Embryo. RANK/TNFRSF11A was detected in immersion fixed frozen sections of rat embryo (cartilage primordium, 7 days p. c.) using 10 ug/ml Goat Anti-Mouse RANK/TNFRSF1 1A Antigen Affinity-purified Polyclonal Antibody overnight at 4°C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained with Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue).
Agonist activity. Mouse RANK/TNFRSF11A Antibody Induces Osteoclast Differentiation in Mouse Splenocytes. Mouse RANK/TNFRSF11A Affinity-purified Polyclonal Antibody induces osteoclast differentiation on mouse splenocytes in the presence of 20 ng/ml recombinant mouse MCSF in a dose dependent manner, as measured by the tartrate-resistant acid phosphatase (TRAP) solution assay. The ED50 for this effect is typically 0.1-0.4 ug/ml.