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Anti-TNF Alpha Antibody (clone 52B83) IHC-plus™ LS-B2123

Note: This antibody replaces LS-C18251

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 52B83] (IgG1) to Human TNF Alpha
Human, Monkey, Mouse, Guinea pig
IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry, ELISA
Unconjugated

Details

Human TNF Alpha
Mouse
Human, Monkey, Mouse, Guinea pig (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [52B83]
Purified

Applications

IHC - Paraffin (2.5 µg/ml), IHC - Frozen, Western blot (1:5), Flow Cytometry, ELISA

Specificity and Use

TNF Alpha antibody was raised against human TNFa.
Recognizes human native and recombinant TNF-alpha. Reacts with soluble (17kD) and membrane (26kD) human TNF-alpha. Does not cross-react with TNF-beta, lymphotoxin and receptor bound TNF-alpha.
Immunohistochemistry: LS-B2123 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2123 was determined to be 2.5 ug/ml. Non-specific background staining is observed in connective tissues.

Packaging

PBS, 0.02% sodium azide, 0.1% BSA.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About TNF Alpha

P01375 NM_000594 NP_000585.2

TNF Antibody, APC1 protein Antibody, Cachectin Antibody, DIF Antibody, TNF Alpha Antibody, Tumor necrosis factor-alpha Antibody, TNF-alpha Antibody, TNF-a Antibody, TNFA Antibody, Tumor necrosis factor Antibody, TNF, macrophage-derived Antibody, TNF, monocyte-derived Antibody, TNFSF2 Antibody

Tumor Necrosis Factor (TNF) is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. TNF is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.

Immunohistochemistry

Immunohistochemistry
Anti-TNF Alpha antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2123 concentration 5 ug/ml.
 

Requested From: United States
Date Requested: 5/30/2015

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