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Catalog Number Size Price
LS-C154152-100 100 µg (1 mg/ml) $567 
TNF Alpha Antibody - Anti-Swine TNFa Antibody - Western Blot. Western blot of protein-A purified anti-swine TNFa antibody shows detection of recombinant swine TNFa at 16.9 kD, raised in yeast. The identity of the faint band at 135 kD is not known. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 1 ug/mL primary antibody overnight at 4C. After washing, membrane was probed with IRDye800 Conjugated Goat Anti-Rabbit IgG ( for 45 min at room temperature.

Polyclonal Rabbit anti‑Pig TNF Alpha Antibody (Lyophilized, WB) LS‑C154152

Polyclonal Rabbit anti‑Pig TNF Alpha Antibody (Lyophilized, WB) LS‑C154152

Antibody:
TNF Alpha Rabbit anti-Pig Polyclonal (Lyophilized) Antibody
Application:
WB
Reactivity:
Pig
Format:
Unconjugated, Lyophilized
Price
Catalog Number
$567
LS-C154152-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
TNF Alpha Rabbit anti-Pig Polyclonal (Lyophilized) Antibody
Application:
WB
Reactivity:
Pig
Format:
Unconjugated, Lyophilized

Specifications

Description
TNF Alpha antibody LS-C154152 is an unconjugated rabbit polyclonal antibody to pig TNF Alpha. Validated for WB.
Target
Pig TNF Alpha
Synonyms
TNF | APC1 protein | Cachectin | DIF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
Host
Rabbit
Reactivity
Pig (tested or 100% immunogen sequence identity)
Predicted
Baboon (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Lyophilized
Immunogen
This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to 154 amino acids of mature swine TNFa.
Specificity
A BLAST analysis was used to suggest cross-reactivity with TNFa from swine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against TNFa from baboon based on 90% homology. Cross-reactivity with TNFa from sources other than swine has not been determined.
Applications
  • Western blot (1:1000)
Usage
This protein A purified antibody is suitable for Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 16.9 kD in size corresponding to swine TNFa by western blotting in the appropriate cell lysate or extract.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TNF Alpha
P01375 NM_000594 NP_000585.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/17/2024