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Anti-TNF Alpha Antibody LS-C104816

Note: This antibody replaces LS-C54302, LS-C42070, LS-C39988

Ordering

Wt. Vol. Conc. Price
50 µg - - $285
100 µg - - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular TNF Alpha Antibodies

Anti-TNF Alpha Antibody (clone 52B83) IHC-plus™ LS-B2123
Mouse Monoclonal [clone 52B83] (IgG1) to Human TNF Alpha
Human, Monkey, Mouse, Guinea pig
IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry, ELISA
Unconjugated
Immunohistochemistry Image
Anti-TNF Alpha Antibody (clone M 1 C4) IHC-plus™ LS-B6067
Mouse Monoclonal [clone M 1 C4] (IgG1,k) to Human TNF Alpha
Human, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Mouse TNF Alpha
Mouse
IHC, Western blot, ELISA, Neutralization (applications tested for the base form of this product only)
Unconjugated

Details

Mouse TNF Alpha
Rabbit
Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin.
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • Western blot
  • ELISA (0.5 - 2 µg/ml)
  • Neutralization (10 - 15 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

TNF Alpha antibody was raised against e.coli derived recombinant Murine TNF-alpha.
Mouse TNF-alpha
Immunohistochemistry: This antibody stained colchicine injected mouse brain (hippocampus CA1 region) tissue. The primary antibody was incubated at 1 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Murine TNF-alpha (0.5 ng/ml), a concentration of 0.04-0.05 ug/ml of this antibody is required. ELISA: To detect Murine TNF-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine TNF-alpha (LS-C104785) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine TNF-alpha. Western Blot: To detect Murine TNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine TNF-alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About TNF Alpha

P01375 NM_000594 NP_000585.2

TNF Antibody, APC1 protein Antibody, Cachectin Antibody, DIF Antibody, TNF Alpha Antibody, Tumor necrosis factor-alpha Antibody, TNF-alpha Antibody, TNF-a Antibody, TNFA Antibody, Tumor necrosis factor Antibody, TNF, macrophage-derived Antibody, TNF, monocyte-derived Antibody, TNFSF2 Antibody

TNF Alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.

Immunohistochemistry

Immunohistochemistry
IHC of TNF Alpha / TNF antibody LS-C104816. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/9/2016

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