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Anti-TNF Alpha Antibody LS-C104718

Note: This antibody replaces LS-C54300, LS-C18314

Ordering

Wt. Vol. Conc. Price
50 µg - - $285
100 µg - - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular TNF Alpha Antibodies

Anti-TNF Alpha Antibody (clone 52B83) IHC-plus™ LS-B2123
Mouse Monoclonal [clone 52B83] (IgG1) to Human TNF Alpha
Human, Monkey, Mouse, Guinea pig
IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry, ELISA
Unconjugated
Immunohistochemistry Image
Anti-TNF Alpha Antibody (clone M 1 C4) IHC-plus™ LS-B6067
Mouse Monoclonal [clone M 1 C4] (IgG1,k) to Human TNF Alpha
Human, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human TNF Alpha
Human
IHC - Paraffin, Western blot, ELISA, Neutralization, Sandwich ELISA
Unconjugated

Details

Human TNF Alpha
Goat
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Western blot
  • ELISA
  • Neutralization (12 - 14 µg/ml)
  • Sandwich ELISA

Specificity and Use

TNF Alpha antibody was raised against e.coli derived recombinant Human TNF-alpha.
Human TNF-alpha
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 2.5 ug/ml-5 ug/ml with a two-hour incubation at room temperature or an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. This antibody was also tested in an HRP-biotin labeling detection system. Heat induced antigen retrieval with a pH 6 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human TNF-alpha (0.25 ng/ml), a concentration of 0.012-0.014 ug/ml of this antibody is required. ELISA: To detect Human TNF-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TNF-alpha (LS-C104788) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human TNF-alpha. Western Blot: To detect Human TNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human TNF-alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About TNF Alpha

P01375 NM_000594 NP_000585.2

TNF Antibody, APC1 protein Antibody, Cachectin Antibody, DIF Antibody, TNF Alpha Antibody, Tumor necrosis factor-alpha Antibody, TNF-alpha Antibody, TNF-a Antibody, TNFA Antibody, Tumor necrosis factor Antibody, TNF, macrophage-derived Antibody, TNF, monocyte-derived Antibody, TNFSF2 Antibody

TNF Alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.

Immunohistochemistry

Immunohistochemistry
IHC of TNF Alpha / TNF antibody LS-C104718

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of TNF Alpha / TNF antibody LS-C104718

Requested From: United States
Date Requested: 12/10/2016

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