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Anti-TNF Alpha Antibody LS-C59380

Ordering

Wt. Vol. Conc. Price
- 1000 µl - $1,110
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular TNF Alpha Antibodies

Anti-TNF Alpha Antibody (clone 52B83) IHC-plus™ LS-B2123
Mouse Monoclonal [clone 52B83] (IgG1) to Human TNF Alpha
Human, Monkey, Mouse, Guinea pig
IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry, ELISA
Unconjugated
Immunohistochemistry Image
Anti-TNF Alpha Antibody (clone M 1 C4) IHC-plus™ LS-B6067
Mouse Monoclonal [clone M 1 C4] (IgG1,k) to Human TNF Alpha
Human, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human TNF Alpha
Human, Primate
IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA, Radioimmunoassay
Unconjugated

Details

Human TNF Alpha
Rabbit
Human, Primate (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Antiserum
Unmodified

Applications

  • IHC - Paraffin
  • ICC
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
  • ELISA
  • Radioimmunoassay

Specificity and Use

TNF Alpha antibody was raised against recombinant human TNFa produced in E. coli.
Mature 17000 MW human TNFa and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFa in the same formats using similar dilutions. The antibody does not recognize human TNGO (lymphotoxin). This antiserum will recognize the cell-bound precursor of TNFa as a.
This antiserum against Human TNFa has been tested for use in neutralizations, ELISA, immunohistochemistry, radioimmunoassay, immunoprecipitation, immunohistochemistry and immunoblotting. It recognizes the 17000 MW TNFa. Reactivity in other immunoassays is unknown.

Packaging

Long term: -20°C; Short term: -20°C
For research use only.

About TNF Alpha

P01375 NM_000594 NP_000585.2

TNF Antibody, APC1 protein Antibody, Cachectin Antibody, DIF Antibody, TNF Alpha Antibody, Tumor necrosis factor-alpha Antibody, TNF-alpha Antibody, TNF-a Antibody, TNFA Antibody, Tumor necrosis factor Antibody, TNF, macrophage-derived Antibody, TNF, monocyte-derived Antibody, TNFSF2 Antibody

TNF Alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.

Immunohistochemistry

Immunohistochemistry
Anti-Human TNFa Antibody - Immunohistochemistry. Immunohistochemistry using polyclonal TNFa antibody showing staining of formalin/PFA-fixed paraffin-embedded sections of human artery tissue sections. Sections were fixed in formaldehyde and subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0). Slides were blocked for ten minutes with 1.5% serum. Primary antibody was diluted 1:100 and incubated with samples for 24 hours at 4°C. HRP-conjugated goat anti-rabbit antibody was used as the secondary antibody.

Immunofluorescence

Immunofluorescence
Anti-Human TNFa Antibody - Immunohistochemistry. Fluorescent immunohistochemistry showing staining of human colon by anti-TNF alpha (formalin/PFA-fixed paraffin-embedded sections). Samples were formaldehyde-fixed, then blocked in 10% serum for 2 hours at 20°C. The primary antibody was diluted 1:100 and incubated with the sample for 2 hours at 20°C. Alexa Fluor 680 goat polyclonal secondary antibody was used diluted 1:5000.

Western blot

Western blot
Anti-Human TNF-a Antibody - Western Blot. Western blot of Anti-Human TNF-a (RABBIT) Antibody. Membrane blocked in 1% BSA-TBS-T 30 min RT, Rb-a-TNF alpha added at 1:1000 in 1% BSA-TBS-T o/n 4C, DyLight 649 Gt-a-Rb added at 1:20000 in MB-070 30 min RT.
 

Requested From: United States
Date Requested: 12/8/2016

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