Bovine TNF Alpha

Mouse

Bovine (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

CC327

Unconjugated. Also available conjugated with Biotin.

Protein G purified

Unmodified

Recombinant bovine TNF alpha.

Recognizes bovine TNF alpha, a 17.5kD cytokine, expressed by many different stimulated cell types including monocytes, macrophages, endothelial cells, fibroblasts and both T and B-lymphocytes. The production of TNF alpha is induced by a variety of factors, dependant upon cell type and includes bacterial toxins, IL-1, PDGF, IFN-beta, NGF, Oncostatin M and viral infections. The presence of TNF alpha is responsible for diverse immunomodulatory, anti-tumor and toxic effects and under certain conditions is also capable of self-stimulation and inhibition. This reagent may be used as a capture antibody in ELISA assays for bovine TNF alpha in combination with MCA2335B as detection reagent.

• Flow Cytometry
• ELISA

Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul. Membrane permeabilization is required for this application. ELISA: This reagent may be used as a capture antibody in sandwich ELISA assays for bovine and sheep TNF alpha in combination with LS-C44702 as detection reagent.

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P01375 NCBI: NM_000594 NP_000585.2