Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse, Rat (tested or 100% immunogen sequence identity)
IHC - Paraffin
IHC - Frozen
Western blot (1:10)
Flow Cytometry (1:10)
Specificity and Use
TNF Alpha antibody was raised against mouse TNF-alpha.
Recognizes both mouse natural and recombinant TNF-alpha and recognizes membrane and receptor bound TNF-alpha. Shows neutralizing activity. Species cross-reactivity: rat.
Suitable for use in Immunohistochemistry, Flow Cytometry, ELISA, Western Blot and Neutralization. Immunohistochemistry (frozen and paraffin sections): 1:10. Flow Cytometry: 1:10. Western Blot: 1:10. Biological assays: Remove sodium azide and sterile filter.
PBS, 0.1% BSA
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
TNF Alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.