Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat Monoclonal [clone MP6-XT22] (IgG1,k) to Mouse TNF Alpha
Western blot, Sandwich ELISA
Mouse TNF Alpha
Mouse (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [MP6-XT22]
Specificity and Use
TNF Alpha antibody was raised against e. coli-expressed recombinant mouse TNF-alpha
Useful as a capture antibody in a sandwich ELISA for quantifying mouse TNF-alpha protein levels. Purified antibody should be paired with a suitably labeled detection antibody as the detection antibody, with purified recombinant mouse TNF-alpha as the standard. Also useful for Western blot under reducing conditions.
Borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added.
TNF Alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.