Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat Monoclonal [clone V1q] (IgD) to Mouse TNF Alpha
Flow Cytometry, Functional Assay
Mouse Monoclonal [clone MAb1] (IgG1,k) to Human TNF Alpha
IHC - Paraffin, ELISA, ELISPOT, Sandwich ELISA
Human TNF Alpha
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [MAb1]
IHC - Paraffin (20 µg/ml)
Specificity and Use
TNF Alpha antibody was raised against human TNF
The MAb1 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human TNF-alpha (TNF-a) in combination with the biotin MAb11 (LS-C106694) antibody for detection and recombinant human TNFa as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 ug/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate.
Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer.
TNF Alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.