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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TNF Alpha Antibody (clone M 1 C4) IHC‑plus™ LS‑B6067
Note: This antibody replaces LS-C133961
TNF Alpha antibody LS-B6067 is an unconjugated mouse monoclonal antibody to TNF Alpha from human and rat. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B6067-50
50 µg (0.45 mg/ml)
Unavailable

Popular TNF Alpha Products

Antibody
Species: Mouse
Applications: Western blot
Anti-TNF Alpha antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2123 concentration 5 ug/ml.
Species: Human, Monkey, Mouse, Guinea pig
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry, ELISA
Antibody
Species: Human, Monkey, Cynomolgus
Applications: Western blot, ELISA
Antibody
Species: Mouse, Hamster
Applications: Flow Cytometry, Functional Assay

Product Description

TNF Alpha antibody LS-B6067 is an unconjugated mouse monoclonal antibody to TNF Alpha from human and rat. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About TNF Alpha
TNF Alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. P01375 NM_000594 NP_000585.2

TNF Antibody, APC1 protein Antibody, Cachectin Antibody, DIF Antibody, TNF Alpha Antibody, Tumor necrosis factor-alpha Antibody, TNF-alpha Antibody, TNF-a Antibody, TNFA Antibody, Tumor necrosis factor Antibody, TNF, macrophage-derived Antibody, TNF, monocyte-derived Antibody, TNFSF2 Antibody


Specifications

Target
Human TNF Alpha
Host
Mouse
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [M 1 C4]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:1000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
TNF Alpha antibody was raised against tNF (AAH28148, 1 a.a. ~ 234 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human TNF
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using tissue lysates and the recombinant protein used as the immunogen.
Presentation
PBS, pH 7.4
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-TNF Alpha antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6067 concentration 5 ug/ml.
Anti-TNF Alpha antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6067 concentration 5 ug/ml.

Immunohistochemistry

Anti-TNF Alpha antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6067 concentration 5 ug/ml.
Anti-TNF Alpha antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6067 concentration 5 ug/ml.

Western blot

TNF monoclonal antibody, LS-B6067. Western blot of TNF expression in rat liver.
TNF monoclonal antibody, LS-B6067. Western blot of TNF expression in rat liver.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged TNF is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged TNF is approximately 0.3 ng/ml as a capture antibody.

Immunohistochemistry

Anti-TNF Alpha antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6067 concentration 5 ug/ml.
Anti-TNF Alpha antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6067 concentration 5 ug/ml.

Immunohistochemistry

Anti-TNF Alpha antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6067 concentration 5 ug/ml.
Anti-TNF Alpha antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6067 concentration 5 ug/ml.

Western blot

TNF monoclonal antibody, LS-B6067. Western blot of TNF expression in rat liver.
TNF monoclonal antibody, LS-B6067. Western blot of TNF expression in rat liver.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged TNF is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged TNF is approximately 0.3 ng/ml as a capture antibody.

Immunohistochemistry

Anti-TNF Alpha antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6067 concentration 5 ug/ml.
Anti-TNF Alpha antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6067 concentration 5 ug/ml.

Immunohistochemistry

Anti-TNF Alpha antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6067 concentration 5 ug/ml.
Anti-TNF Alpha antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6067 concentration 5 ug/ml.

Western blot

TNF monoclonal antibody, LS-B6067. Western blot of TNF expression in rat liver.
TNF monoclonal antibody, LS-B6067. Western blot of TNF expression in rat liver.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged TNF is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged TNF is approximately 0.3 ng/ml as a capture antibody.

Immunohistochemistry

Anti-TNF Alpha antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6067 concentration 5 ug/ml.
Anti-TNF Alpha antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6067 concentration 5 ug/ml.

Immunohistochemistry

Anti-TNF Alpha antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6067 concentration 5 ug/ml.
Anti-TNF Alpha antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6067 concentration 5 ug/ml.

Western blot

TNF monoclonal antibody, LS-B6067. Western blot of TNF expression in rat liver.
TNF monoclonal antibody, LS-B6067. Western blot of TNF expression in rat liver.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged TNF is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged TNF is approximately 0.3 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/18/2018

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