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Anti-TNF Alpha Antibody (Biotin) LS-C44702


Wt. Vol. Conc. Price
250 µg - 1 mg/ml $445
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular TNF Alpha Antibodies

Anti-TNF Alpha Antibody (clone 52B83) IHC-plus™ LS-B2123
Mouse Monoclonal [clone 52B83] (IgG1) to Human TNF Alpha
Human, Monkey, Mouse, Guinea pig
IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry, ELISA
Immunohistochemistry Image
Anti-TNF Alpha Antibody (clone M 1 C4) IHC-plus™ LS-B6067
Mouse Monoclonal [clone M 1 C4] (IgG1,k) to Human TNF Alpha
Human, Rat
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG2a) to Bovine TNF Alpha
Bovine, Sheep
Flow Cytometry, ELISA (applications tested for the base form of this product only)
Biotin Conjugated


Bovine TNF Alpha
Bovine, Sheep (tested or 100% immunogen sequence identity)
IgG2a Monoclonal
Protein G purified


  • Flow Cytometry (1:10 - 1:100)
  • ELISA (1 - 5 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

TNF Alpha antibody was raised against recombinant bovine TNF alpha.
Recognizes bovine TNF alpha, a 17.5kD cytokine, expressed by many different stimulated cell types including monocytes, macrophages, endothelial cells, fibroblasts and both T and B-lymphocytes. The production of TNF alpha is induced by a variety of factors, dependant upon cell type and includes bacterial toxins, IL-1, PDGF, IFN-beta, NGF, Oncostatin M and viral infections. The presence of TNF alpha is responsible for diverse immunomodulatory, anti-tumor and toxic effects and under certain conditions is also capable of self-stimulation and inhibition. This biotin conjugate may be used as a detection antibody in ELISA assays for bovine TNF alpha in combination with MCA2334 as capture reagent.
The applications listed have been tested for the base form of this product. Alternate forms, such as conjugated, azide-free, or ready-to-use, have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul. Membrane permeabilization is required for this application. ELISA: This biotin conjugate may be used as a detection antibody in sandwich ELISA assays for bovine TNF alpha in combination with LS-C44701 as capture reagent.


PBS, 0.09% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About TNF Alpha

P01375 NM_000594 NP_000585.2

TNF Antibody, APC1 protein Antibody, Cachectin Antibody, DIF Antibody, TNF Alpha Antibody, Tumor necrosis factor-alpha Antibody, TNF-alpha Antibody, TNF-a Antibody, TNFA Antibody, Tumor necrosis factor Antibody, TNF, macrophage-derived Antibody, TNF, monocyte-derived Antibody, TNFSF2 Antibody

TNF Alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.

Requested From: United States
Date Requested: 10/22/2016

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