Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Bovine (tested or 100% immunogen sequence identity)
Western blot (1 - 10 µg/ml)
ELISA (1 - 10 µg/ml)
IHC - Paraffin
Specificity and Use
TNF Alpha antibody was raised against recombinant bovine TNF alpha.
Recognizes bovine tumor necrosis factor alpha (TNF alpha). TNF alpha is produced by a range of cells including macrophages, monocytes, T and B cells, neutrophils and endothelial cells. TNF alpha has a key regulatory role in the bovine immune response, where it is involved in modulating the expression of numerous cytokines, growth factors, inflammatory mediators and acute phase proteins.
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Proteome of the early embryo-maternal dialogue in the cattle uterus. Mu[Character f1]oz M, Corrales FJ, Caama[Character f1]o JN, D[Character ed]ez C, Trigal B, Mora MI, Mart[Character ed]n D, Carrocera S, G[Character f3]mez E. Journal of proteome research. 2012 Feb;11:751-66. [Full Text Article]
Sulforaphane epigenetically regulates innate immune responses of porcine monocyte-derived dendritic cells induced with lipopolysaccharide. Qu X, Pröll M, Neuhoff C, Zhang R, Cinar MU, Hossain MM, Tesfaye D, Große-Brinkhaus C, Salilew-Wondim D, Tholen E, Looft C, Hölker M, Schellander K, Uddin MJ. PloS one. 2015 10:e0121574. (WB; Bovine)[Full Text Article]
TNF Alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.