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Anti-TNF Alpha Antibody (aa78-232) LS-C150284

Catalog	Size	Price
LS-C150284-25	25 µg	Unavailable
LS-C150284-100	100 µg	Unavailable

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Antibody:	Goat Polyclonal (IgG) to Pig TNF Alpha
Reactivity:	Pig
Application:	ICC, Western blot, Neutralization
Format:	Unconjugated

Cytotoxicity Induced by TNF- alpha and Neutralization by Porcine TNF- alpha Antibody. Recombinant Porcine TNF- alpha induces cytotoxicity in the the PK-15 porcine kidney epithelial cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Porcine TNF- alpha (0.05 ng/ml) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine TNF- alpha Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.2-0.8 ug/ml in the presence of the metabolic inhibitor actinomycin D (1 ug/ml).

Details

Target:	Pig TNF Alpha
Host:	Goat
Reactivity:	Pig (tested or 100% immunogen sequence identity)
Clonality:	IgG Polyclonal
Conjugations:	Unconjugated
Purification:	Immunoaffinity purified
Modifications:	Unmodified

Applications

Approved:

ICC (5 - 15 µg/ml)
Western blot (0.1 µg/ml)
Neutralization

Specificity and Use

Immunogen:	TNF Alpha antibody was raised against e. coli-derived recombinant porcine TNFalpha Arg78-Leu232 Accession # P23563.
Epitope:	aa78-232
Specificity:	Detects porcine TNF alpha in direct ELISAs and Western blots.
Usage:	Western Blot, 0.1 µg/ml, Recombinant Porcine TNF-alpha. Immunocytochemistry, 5-15 µg/ml, Immersion fixed porcine peripheral blood mononuclear cells. Neutralization, Measured by its ability to neutralize TNF-alpha-induced cytotoxicity in the PK-15 porcine kidney epithelial cell line. The Neutralization Dose (ND50) is typically 0.2-0.8 µg/ml in the presence of 0.05 ng/ml Recombinant Porcine TNF-alpha and 1 µg/ml actinomycin D.

Packaging

Presentation:	Lyophilized from 0.2 um filtered solution in PBS, trehalose
Reconstitution:	Sterile PBS. Resuspend to 500 ul
Storage:	Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions:	For research use only.

About TNF Alpha

Uniprot: P01375 NCBI: NM_000594 NP_000585.2

Synonyms: TNF Antibody, APC1 protein Antibody, Cachectin Antibody, DIF Antibody, TNF Alpha Antibody, Tumor necrosis factor-alpha Antibody, TNF-alpha Antibody, TNF-a Antibody, TNFA Antibody, Tumor necrosis factor Antibody, TNF, macrophage-derived Antibody, TNF, monocyte-derived Antibody, TNFSF2 Antibody

Description: TNF Alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.

(More About TNF Alpha)

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Neutralization

Cytotoxicity Induced by TNF- alpha and Neutralization by Porcine TNF- alpha Antibody. Recombinant Porcine TNF- alpha induces cytotoxicity in the the PK-15 porcine kidney epithelial cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Porcine TNF- alpha (0.05 ng/ml) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine TNF- alpha Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.2-0.8 ug/ml in the presence of the metabolic inhibitor actinomycin D (1 ug/ml).

Requested From:
Date Requested: 4/23/2018

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