Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
Products
Antibodies
ELISA and Assay Kits
Research Areas
Infectious Disease
Resources
Purchasing
Reference Material
Contact Us
Locations
Orders Processing,
Shipping & Receiving,
Warehouse
2 Shaker Rd Suites
B001/B101
Shirley, MA 01464
Production Lab
Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036
Telephone Numbers
Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565
Contact Us
Additional Contact Details
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
| Catalog Number | Size | Price |
|---|---|---|
| LS-C391066-100 | 100 µg (1 mg/ml) | $574 |
Monoclonal Mouse anti‑Human TNF Alpha Antibody (clone TNF706 + P/T2, Azide‑free,BSA‑free, aa115‑130, IHC, IF) LS‑C391066
Monoclonal Mouse anti‑Human TNF Alpha Antibody (clone TNF706 + P/T2, Azide‑free,BSA‑free, aa115‑130, IHC, IF) LS‑C391066
Antibody:
TNF Alpha Mouse anti-Human Monoclonal (aa115-130) (Azide-free, BSA-free) (TNF706 + P/T2) Antibody
Application:
IHC, IHC-P, IF, Flo
Reactivity:
Human, Mouse, Rat, Dog, Rabbit, Zebrafish
Format:
Unconjugated, Azide-free, BSA-free
Other formats:
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
TNF Alpha Mouse anti-Human Monoclonal (aa115-130) (Azide-free, BSA-free) (TNF706 + P/T2) Antibody
Application:
IHC, IHC-P, IF, Flo
Reactivity:
Human, Mouse, Rat, Dog, Rabbit, Zebrafish
Format:
Unconjugated, Azide-free, BSA-free
Other formats:
Specifications
Description
TNF Alpha antibody LS-C391066 is an unconjugated mouse monoclonal antibody to TNF Alpha (aa115-130) from human. It is reactive with human, mouse, rat and other species. Validated for Flow, IF and IHC.
Target
Human TNF Alpha
Synonyms
TNF | APC1 protein | Cachectin | DIF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
Host
Mouse
Reactivity
Human, Mouse, Rat, Dog, Rabbit, Zebrafish
(tested or 100% immunogen sequence identity)
Clonality
IgM
Monoclonal
Clone
TNF706 + P/T2
Conjugations
Unconjugated
Purification
PEG purified
Modifications
Azide-free, BSA-free.
Also available Unmodified or Ready-to-Use.
Immunogen
N-Terminus recombinant protein fragment (TNF706) and Synthetic peptide (NGVELRDNQLVVPSEG, aa115-130 of UniProt P01375) were used as the immunogen for the TNF-alpha antibody cocktail. Percent identity by BLAST analysis: Human (100%); Ferret, Sheep, Cat, Goat, Elephant, Dog (81%); Mouse, Rat (69%).
Epitope
aa115-130
Specificity
Human TNF Alpha
Applications
- IHC
- IHC - Paraffin (2 - 4 µg/ml)
- Immunofluorescence (1 - 2 µg/ml)
- Flow Cytometry (0.5 - 1 µg/10E6 cells)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. TNF alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha causes cytolysis of certain transformed cells. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex. Optimal dilution of the TNF-alpha antibody cocktail should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
Presentation
1X PBS
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About TNF Alpha
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Mouse, Hamster
Applications:
Flow Cytometry, Functional Assay
Species:
Human, Rat
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human, Monkey, Mouse, Guinea pig
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry, ELISA
Species:
Mouse
Applications:
IHC, Western blot, Immunoprecipitation, ELISA, Neutralization, Radioimmunoassay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
Date Requested: 4/25/2024
