Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal (IgG1,k) to Human TNC / Tenascin C
ICC, Western blot, ELISA
Human TNC / Tenascin C
Human (tested or 100% immunogen sequence identity)
Protein A purified
Specificity and Use
TNC / Tenascin C antibody was raised against purified human tenascin.
Binds to a recombinant fragment of human tenascin containing the fibronectin type III repeats, numbers 4 and 5.
Immunocytochemistry at 2 ug/ml on U251 cells. Western Blot (Western) at 2 ug/ml to detect whole tenascin under reducing conditions. ELISA: 2 x 10^10 M endpoint dilution. Affinity constant Kd= 2 x 10^10.
0.02 M phosphate, 0.25 M sodium chloride, pH 7.6, 0.1% sodium azide.
Tenascin C (TNC) is the founding member of the gene family. In the embryo it is made by migrating cells like the neural crest; it is also abundant in developing tendons, bone and cartilage. The basic structure is 14 EGF-like repeats towards the N-terminal end, and 8 or more fibronectin-III domains which vary upon species and variant. TNC has anti-adhesive properties, causing cells in tissue culture to become rounded after it is added to the medium.