Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone DB7] (IgG2a) to Human TNC / Tenascin C
IHC - Paraffin, Immunofluorescence, Western blot
Goat Polyclonal to Human TNC / Tenascin C
Human TNC / Tenascin C
Human (tested or 100% immunogen sequence identity)
Monkey, Bovine, Pig (at least 90% immunogen sequence identity)
IHC - Paraffin
Specificity and Use
Synthetic peptide C-AEKGPQKSSTITAK from an internal region of human TNC / Tenascin C (NP_002151.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%); Monkey, Bovine, Pig (93%); Elephant, Panda, Dog, Bat (86%).
LS-E27068 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C112660. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Human TNC / Tenascin C.
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Preliminary experiments in Human Skeletal Muscle, Placenta and Uterus lysates gave no specific signal but low background (at antibody concentration up to 1 ug/ml).
Tenascin C (TNC) is the founding member of the gene family. In the embryo it is made by migrating cells like the neural crest; it is also abundant in developing tendons, bone and cartilage. The basic structure is 14 EGF-like repeats towards the N-terminal end, and 8 or more fibronectin-III domains which vary upon species and variant. TNC has anti-adhesive properties, causing cells in tissue culture to become rounded after it is added to the medium.