Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Protein G purified
Specificity and Use
TMPRSS5 antibody was raised against nS0-derived rhSpinesin.
Selected for its ability to detect human Spinesin in direct ELISAs and Western Blots.
Immunoprecipitation: This antibody was used at a concentration of 25 ug/ml to immunoprecipitate rhSpinesin from conditioned cell culture medium. The recovered rhSpinesin can be detected in Western blots using a polyclonal antibody. Western Blot: This antibody can be used at 1-2 ug/ml with the appropriate secondary reagents to detect human Spinesin. Using a colorimetric detection system, the detection limit for rhSpinesin is approximately 25 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. For Western blot under reducing conditions, AF2495 and BAF2495 are recommended. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human Spinesin. The detection limit for rhSpinesin is approximately 20 ng/well.