Human TMPRSS5

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated

Protein G purified

Unmodified

Recombinant protein corresponding to Tyr27-Leu457 from human Spinesin expressed in NS0 cells.

Selected for its ability to detect human Spinesin in direct ELISAs and Western Blots.

• Western blot
• Immunoprecipitation
• ELISA

Immunoprecipitation: This antibody was used at a concentration of 25 ug/ml to immunoprecipitate rhSpinesin from conditioned cell culture medium. The recovered rhSpinesin can be detected in Western blots using a polyclonal antibody. Western Blot: This antibody can be used at 1-2 ug/ml with the appropriate secondary reagents to detect human Spinesin. Using a colorimetric detection system, the detection limit for rhSpinesin is approximately 25 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. For Western blot under reducing conditions, AF2495 and BAF2495 are recommended. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human Spinesin. The detection limit for rhSpinesin is approximately 20 ng/well.

Lyophilized

Reconstitute with 200ul sterile PBS

Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9H3S3 NCBI: NM_030770 NP_110397.2