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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TMEM142A / ORAI1 Antibody (N‑Terminus) LS‑C19679
Note: This antibody replaces LS-C37893, LS-C33447
ORAI1 antibody LS-C19679 is an unconjugated rabbit polyclonal antibody to ORAI1 (TMEM142A) from human and mouse. Validated for IHC and WB.
Catalog
Size
Price
LS-C19679-100
100 µg (1 mg/ml)
Unavailable

Popular TMEM142A / ORAI1 Products

Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 2.5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, Western blot
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Western blot
Antibody (0.03 ug/ml) staining of Human Placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Species: Human, Dog, Pig, Rabbit
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 2.5 ug/ml.
Species: Human
Applications: IHC, Western blot
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with ORAI1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

ORAI1 antibody LS-C19679 is an unconjugated rabbit polyclonal antibody to ORAI1 (TMEM142A) from human and mouse. Validated for IHC and WB.
About TMEM142A / ORAI1
Ca2+ release-activated Ca2+ (CRAC) channel subunit which mediates Ca2+ influx following depletion of intracellular Ca2+ stores and channel activation by the Ca2+ sensor, STIM1. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. Q96D31 NM_032790 NP_116179.2

ORAI1 Antibody, CRACM1 Antibody, ORAT1 Antibody, Protein orai-1 Antibody, TMEM142A Antibody, Transmembrane protein 142A Antibody


Specifications

Target
Human TMEM142A / ORAI1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E30312 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-C19679. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.
Presentation
PBS, 0.02% sodium azide.
Storage
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 10 µg/mL.
Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 10 µg/mL.

Immunohistochemistry

Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 10 ug/ml.
Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 10 ug/ml.

Immunohistochemistry

Immunohistochemistry of ORAI1 in human ovary tissue with ORAI1 antibody at 2.5 µg/ml.
Immunohistochemistry of ORAI1 in human ovary tissue with ORAI1 antibody at 2.5 µg/ml.

Immunofluorescence

Immunofluorescence of ORAI1 in Human Spleen cells with ORAI1 antibody at 20 µg/mL.
Immunofluorescence of ORAI1 in Human Spleen cells with ORAI1 antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of ORAI1 in Human Spleen cells with ORAI1 antibody at 20 ug/ml.
Immunofluorescence of ORAI1 in Human Spleen cells with ORAI1 antibody at 20 ug/ml.

Western blot

Western blot analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at 1 µg/mL in the (A) absence or (B) presence of blocking peptide.
Western blot analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at 1 µg/mL in the (A) absence or (B) presence of blocking peptide.

Western blot

Western blot of ORAI1 in human ovary tissue lysate with ORAI1 antibody at 1 ug/ml in the (A) absence or (B) presence of blocking peptide.
Western blot of ORAI1 in human ovary tissue lysate with ORAI1 antibody at 1 ug/ml in the (A) absence or (B) presence of blocking peptide.

Immunohistochemistry

Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 10 µg/mL.
Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 10 µg/mL.

Immunohistochemistry

Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 10 ug/ml.
Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 10 ug/ml.

Immunohistochemistry

Immunohistochemistry of ORAI1 in human ovary tissue with ORAI1 antibody at 2.5 µg/ml.
Immunohistochemistry of ORAI1 in human ovary tissue with ORAI1 antibody at 2.5 µg/ml.

Immunofluorescence

Immunofluorescence of ORAI1 in Human Spleen cells with ORAI1 antibody at 20 µg/mL.
Immunofluorescence of ORAI1 in Human Spleen cells with ORAI1 antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of ORAI1 in Human Spleen cells with ORAI1 antibody at 20 ug/ml.
Immunofluorescence of ORAI1 in Human Spleen cells with ORAI1 antibody at 20 ug/ml.

Western blot

Western blot analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at 1 µg/mL in the (A) absence or (B) presence of blocking peptide.
Western blot analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at 1 µg/mL in the (A) absence or (B) presence of blocking peptide.

Western blot

Western blot of ORAI1 in human ovary tissue lysate with ORAI1 antibody at 1 ug/ml in the (A) absence or (B) presence of blocking peptide.
Western blot of ORAI1 in human ovary tissue lysate with ORAI1 antibody at 1 ug/ml in the (A) absence or (B) presence of blocking peptide.

Immunohistochemistry

Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 10 µg/mL.
Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 10 µg/mL.

Immunohistochemistry

Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 10 ug/ml.
Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 10 ug/ml.

Immunohistochemistry

Immunohistochemistry of ORAI1 in human ovary tissue with ORAI1 antibody at 2.5 µg/ml.
Immunohistochemistry of ORAI1 in human ovary tissue with ORAI1 antibody at 2.5 µg/ml.

Immunofluorescence

Immunofluorescence of ORAI1 in Human Spleen cells with ORAI1 antibody at 20 µg/mL.
Immunofluorescence of ORAI1 in Human Spleen cells with ORAI1 antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of ORAI1 in Human Spleen cells with ORAI1 antibody at 20 ug/ml.
Immunofluorescence of ORAI1 in Human Spleen cells with ORAI1 antibody at 20 ug/ml.

Western blot

Western blot analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at 1 µg/mL in the (A) absence or (B) presence of blocking peptide.
Western blot analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at 1 µg/mL in the (A) absence or (B) presence of blocking peptide.

Western blot

Western blot of ORAI1 in human ovary tissue lysate with ORAI1 antibody at 1 ug/ml in the (A) absence or (B) presence of blocking peptide.
Western blot of ORAI1 in human ovary tissue lysate with ORAI1 antibody at 1 ug/ml in the (A) absence or (B) presence of blocking peptide.

Immunohistochemistry

Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 10 µg/mL.
Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 10 µg/mL.

Immunohistochemistry

Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 10 ug/ml.
Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 10 ug/ml.

Immunohistochemistry

Immunohistochemistry of ORAI1 in human ovary tissue with ORAI1 antibody at 2.5 µg/ml.
Immunohistochemistry of ORAI1 in human ovary tissue with ORAI1 antibody at 2.5 µg/ml.

Immunofluorescence

Immunofluorescence of ORAI1 in Human Spleen cells with ORAI1 antibody at 20 µg/mL.
Immunofluorescence of ORAI1 in Human Spleen cells with ORAI1 antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of ORAI1 in Human Spleen cells with ORAI1 antibody at 20 ug/ml.
Immunofluorescence of ORAI1 in Human Spleen cells with ORAI1 antibody at 20 ug/ml.

Western blot

Western blot analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at 1 µg/mL in the (A) absence or (B) presence of blocking peptide.
Western blot analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at 1 µg/mL in the (A) absence or (B) presence of blocking peptide.

Western blot

Western blot of ORAI1 in human ovary tissue lysate with ORAI1 antibody at 1 ug/ml in the (A) absence or (B) presence of blocking peptide.
Western blot of ORAI1 in human ovary tissue lysate with ORAI1 antibody at 1 ug/ml in the (A) absence or (B) presence of blocking peptide.


Requested From: 
Date Requested: 6/17/2018

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