Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 1E4] (IgG2a) to Human TMEFF2
Immunofluorescence, Western blot, Flow Cytometry
Mouse Monoclonal [clone J4B6] (IgG2b) to Human TMEFF2
Western blot, ELISA
Human (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [J4B6]
Protein G purified
Western blot (1:500 - 1:1000)
IHC - Paraffin
Specificity and Use
TMEFF2 antibody was raised against recombinant human TMEFF2 (41-150aa) purified from E. coli. NCBI Accession No.: NP_057276.
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500-1:1000.
PBS, pH 7.4, 0.1% sodium azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
TMEFF2 is a member of the tomoregulin family of transmembrane proteins. This protein has been shown to function as both an oncogene and a tumor suppressor depending on the cellular context and may regulate prostate cancer cell invasion. Multiple soluble forms of this protein have been identified that arise from both an alternative splice variant and ectodomain shedding. Additionally, this gene has been found to be hypermethylated in multiple cancer types.
Cell lysates of Mouse brain and HeLa (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human TMEFF2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.