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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TLR9 Antibody (C‑Terminus) IHC‑plus™ LS‑B756
Note: This antibody replaces LS-C26899
TLR9 antibody LS-B756 is an unconjugated rabbit polyclonal antibody to TLR9 from human and mouse. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B756-50
50 µg (1 mg/ml)
Unavailable

Popular TLR9 Products

Anti-TLR9 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B688 concentration 10 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Anti-TLR9 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2341 concentration 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry
Staining of human TLR9 transfected HEK293 cells with 1.0 ug of PE Rat IgG2a isotype control (open histogram) or 1.0 ug of PE eB72-1665 (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Flow Cytometry
Anti-TLR9 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6184 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot

Product Description

TLR9 antibody LS-B756 is an unconjugated rabbit polyclonal antibody to TLR9 from human and mouse. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About TLR9
TLR9 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Q9NR96 NM_017442 NP_059138.1

TLR9 Antibody, CD289 Antibody, CD289 antigen Antibody, Scri2a Antibody, Toll-like receptor 9 Antibody


Specifications

Target
Human TLR9
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
TLR9 antibody was raised against synthetic peptide from human TLR9.
Epitope
C-Terminus
Specificity
16 amino acids near the C-terminus of human TLR9
Usage
Immunohistochemistry: LS-B756 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B756 was determined to be 10 ug/ml.
Presentation
PBS, 0.02% sodium azide.
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-TLR9 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B756 concentration 10 ug/ml.
Anti-TLR9 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B756 concentration 10 ug/ml.

Western blot

Western blot of TLR9 antibody LS-B756 in mouse spleen cell lysate with TLR9 antibody at (A) 0.5 (B) 1 ug/ml
Western blot of TLR9 antibody LS-B756 in mouse spleen cell lysate with TLR9 antibody at (A) 0.5 (B) 1 ug/ml

Immunohistochemistry

Anti-TLR9 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B756 concentration 10 ug/ml.
Anti-TLR9 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B756 concentration 10 ug/ml.

Western blot

Western blot of TLR9 antibody LS-B756 in mouse spleen cell lysate with TLR9 antibody at (A) 0.5 (B) 1 ug/ml
Western blot of TLR9 antibody LS-B756 in mouse spleen cell lysate with TLR9 antibody at (A) 0.5 (B) 1 ug/ml

Immunohistochemistry

Anti-TLR9 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B756 concentration 10 ug/ml.
Anti-TLR9 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B756 concentration 10 ug/ml.

Western blot

Western blot of TLR9 antibody LS-B756 in mouse spleen cell lysate with TLR9 antibody at (A) 0.5 (B) 1 ug/ml
Western blot of TLR9 antibody LS-B756 in mouse spleen cell lysate with TLR9 antibody at (A) 0.5 (B) 1 ug/ml

Immunohistochemistry

Anti-TLR9 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B756 concentration 10 ug/ml.
Anti-TLR9 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B756 concentration 10 ug/ml.

Western blot

Western blot of TLR9 antibody LS-B756 in mouse spleen cell lysate with TLR9 antibody at (A) 0.5 (B) 1 ug/ml
Western blot of TLR9 antibody LS-B756 in mouse spleen cell lysate with TLR9 antibody at (A) 0.5 (B) 1 ug/ml


Requested From: 
Date Requested: 6/19/2018

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