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Anti-TLR9 Antibody (aa268-284, FITC) IHC-plus™ LS-B2839

Note: This antibody replaces LS-C10779, LS-C10792
Catalog Size Price
LS-B2839-50 50 µg (0.5 mg/ml) Unavailable

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100% Guaranteed
Mouse Monoclonal (IgG1) to Human TLR9
Human, Monkey, Mouse, Rat, Dog, Horse
IHC - Paraffin, IHC - Frozen, ICC, Western blot, Flow Cytometry
FITC Conjugated

Details

Human TLR9
Mouse
Human, Monkey, Mouse, Rat, Dog, Horse (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Fluorescein
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (5 - 10 µg/ml)
  • IHC - Frozen (10 - 20 µg/ml)
  • ICC
  • Western blot (1 - 3 µg/ml)
  • Flow Cytometry

Specificity and Use

TLR9 antibody was raised against synthetic peptide corresponding to aa 268-284 in the cytoplasmic portion of human TLR9 (Toll-like Receptor 9).
aa268-284
Recognizes human TLR9. Detects a band of ~120kD by Western blot, may also detect a smaller band of TLR9 isoform B.
Immunohistochemistry: LS-B2839 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2839 was determined to be 5-10 ug/ml. Flow Cytometry (Intracellular): 0.1-2 ug/10^6 cells; cell surface. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

10 mM Tris, 150 mM sodium chloride, 0.05% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Sensitive to light.
For research use only.

About TLR9

Q9NR96 NM_017442 NP_059138.1

TLR9 Antibody, CD289 Antibody, CD289 antigen Antibody, Scri2a Antibody, Toll-like receptor 9 Antibody

TLR9 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity.

Immunohistochemistry

Immunohistochemistry
Anti-TLR9 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2839 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 8/21/2017

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