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MD-2 Complex antibody LS-C10734 is a biotin-conjugated rat monoclonal antibody to mouse MD-2 Complex (TLR4). Validated for Flow, IHC and IP.
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Review This ProductPopular TLR4/MD-2 Complex Products
Species:
Mouse
Applications:
Immunoprecipitation, Flow Cytometry
Species:
Mouse
Applications:
IHC, IHC - Frozen, Immunoprecipitation, Flow Cytometry, Functional Assay
Species:
Mouse
Applications:
IHC, IHC - Frozen, Immunoprecipitation, Flow Cytometry
Species:
Mouse
Applications:
IHC, IHC - Frozen, Immunoprecipitation, Flow Cytometry
Product Description
MD-2 Complex antibody LS-C10734 is a biotin-conjugated rat monoclonal antibody to mouse MD-2 Complex (TLR4). Validated for Flow, IHC and IP.
Specifications
Target
Mouse TLR4/MD-2 Complex
Host
Rat
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Conjugations
Biotin
Purification
Purified
Modifications
Unmodified
Applications
- IHC
- IHC - Frozen
- Immunoprecipitation
- Flow Cytometry (1:50)
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Specificity
Reacts with the Toll-like receptor 4 (TLR4, CD284). Reacts preferentially, especially in Flow Cytometry, with mouse TLR4 that is associated with MD-2. This antibody is a TLR4 function-blocking antibody that is useful for studies on the role of TLR4 as a receptor for LPS induced cytokine production by TLR4 bearing cells. The antibody was shown to coprecipitate MD-2 (30kD) with TLR4 (100kD). The antibody recognizes the mouse TLR4/MD-2 complex and is not useful for detection of TLR4 (CD284) alone.
Usage
Can be used for Flow Cytometry, Immunoprecipitation and Immunohistology on frozen sections. The antibody recognizes the mouse TLR4/MD-2 complex and is not useful for detection of TLR4 (CD284) alone. Immunohistology: 1:50. Flow Cytometry: 1:50. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 0.02% Sodium Azide, 0.1% BSA
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
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Requested From: United States
Date Requested: 10/21/2018
Date Requested: 10/21/2018
