MD-2 Complex antibody LS-C10734 is a biotin-conjugated rat monoclonal antibody to mouse MD-2 Complex (TLR4). Validated for Flow, IHC and IP.

Mouse TLR4/MD-2 Complex

Rat

Mouse (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

Biotin

Purified

Unmodified

• IHC
• IHC - Frozen
• Immunoprecipitation
• Flow Cytometry (1:50)

Reacts with the Toll-like receptor 4 (TLR4, CD284). Reacts preferentially, especially in Flow Cytometry, with mouse TLR4 that is associated with MD-2. This antibody is a TLR4 function-blocking antibody that is useful for studies on the role of TLR4 as a receptor for LPS induced cytokine production by TLR4 bearing cells. The antibody was shown to coprecipitate MD-2 (30kD) with TLR4 (100kD). The antibody recognizes the mouse TLR4/MD-2 complex and is not useful for detection of TLR4 (CD284) alone.

Can be used for Flow Cytometry, Immunoprecipitation and Immunohistology on frozen sections. The antibody recognizes the mouse TLR4/MD-2 complex and is not useful for detection of TLR4 (CD284) alone. Immunohistology: 1:50. Flow Cytometry: 1:50. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

PBS, 0.02% Sodium Azide, 0.1% BSA

Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.

For research use only.