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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C44224-0.5 0.5 mg $590 
Antibody

TLR4 Antibody (clone HTA125, Low Endotoxin) LS‑C44224

TLR4 Antibody (clone HTA125, Low Endotoxin) LS‑C44224

Antibody:
Mouse Monoclonal to Human TLR4
Application:
WB, IP, Flo
Reactivity:
Human
Format:
Unconjugated, Low Endotoxin
Other formats:
Price
Catalog Number
$590 
LS-C44224-0.5
0.5 mg
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human TLR4
Application:
WB, IP, Flo
Reactivity:
Human
Format:
Unconjugated, Low Endotoxin
Other formats:

Specifications

Description
TLR4 antibody LS-C44224 is an unconjugated mouse monoclonal antibody to human TLR4. Validated for Flow, IP and WB.
Target
Human TLR4
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Clone
HTA125
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Low Endotoxin. Also available Unmodified.
Applications
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry (1:10 - 1:50)
  • Applications tested for the base form of this product only
Failed Applications
  • IHC - Paraffin
Immunogen
Ba/F3 cell line expressing TLR4 (CD284)
Specificity
Recognizes the human Toll like receptor 4 (TLR4) cell surface antigen. TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS. Has been demonstrated to block activation of monocytes with LPS.
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells or 100 ul whole blood. Method sheets are available on request.
Presentation
PBS
Storage
Store at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TLR4
O00206 NM_138554 O00206

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Requested From: United States
Date Requested: 12/13/2019