LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
TLR4 Antibody (FITC) LS‑C44225
Note: This antibody replaces LS-C57652
Antibody
TLR4 antibody LS-C44225 is an FITC-conjugated mouse monoclonal antibody to TLR4 from human, dog, guinea pig and other species. Validated for Flow, IP and WB.
Catalog
Size
Price
LS-C44225-100
100 µg (0.1 mg/ml)
Unavailable

Popular TLR4 Products

Human peripheral blood monocytes stained with HTA125 PE
Species: Human, Guinea pig, Pig
Applications: IHC, IHC - Frozen, Immunofluorescence, Flow Cytometry
Anti-TLR4 antibody LS-A9704 IHC of human placenta, decidua. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin
Anti-TLR4 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2070 concentration 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Anti-TLR4 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2254 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Flow Cytometry, ELISA
Anti-TLR4 antibody IHC of human neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4696 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin

Product Description

TLR4 antibody LS-C44225 is an FITC-conjugated mouse monoclonal antibody to TLR4 from human, dog, guinea pig and other species. Validated for Flow, IP and WB.
About TLR4
Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni2+. Responses triggered by Ni2+ require non-conserved histidines and are, therefore, species-specific. O00206 NM_138554 O00206

TLR4 Antibody, CD284 Antibody, CD284 antigen Antibody, Homolog of Drosophila toll Antibody, TLR-4 Antibody, TOLL Antibody, ARMD10 Antibody, Toll-like receptor 4 Antibody, HToll Antibody


Specifications

Target
Human TLR4
Host
Mouse
Reactivity
Human, Dog, Guinea pig, Pig, Rhesus monkey (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Conjugations
Fluorescein. Also available Unconjugated or conjugated with Biotin, RPE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry (1:1)
  • (applications tested for the base form of this product only)
Immunogen
TLR4 antibody was raised against ba/F3 cell line expressing TLR4 (CD284).
Specificity
Recognizes the human Toll like receptor 4 (TLR4) cell surface antigen. TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS. Has been demonstrated to block activation of monocytes with LPS. XXX XXX recommend the use of MCA2061EL for this purpose.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells or 100 ul whole blood. Method sheets are available on request.
Presentation
PBS, 0.09% sodium azide, 1% BSA.
Storage
4°C or -20°C, Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Requested From: 
Date Requested: 6/19/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number