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Anti-TLR4 Antibody (clone 1E11) IHC-plus™ LS-B2254

Note: This antibody replaces LS-C10724, LS-C149672

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular TLR4 Antibodies

Anti-TLR4 Antibody (Internal) IHC-plus™ LS-A9704
Rabbit Polyclonal to Human TLR4
Human
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-TLR4 Antibody (aa100-200, clone 76B357.1) IHC-plus™ LS-B2070
Mouse Monoclonal [clone 76B357.1] (IgG2b,k) to Human TLR4
Human, Mouse, Rat
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-TLR4 Antibody (clone HTA125, PE, Cy7) LS-C107190
Mouse Monoclonal [clone HTA125] (IgG2a,k) to Human TLR4
Human
Flow Cytometry
PE, Cy7 Conjugated
Flow Cytometry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 1E11] (IgG2a) to Human TLR4
Human
IHC - Paraffin, Flow Cytometry, ELISA
Unconjugated

Details

Human TLR4
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [1E11]
Unconjugated
Purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Flow Cytometry
  • ELISA (1:2000 - 1:4000)

Specificity and Use

TLR4 antibody was raised against recombinant protein corresponding to amino acids 238-609 of human Toll-like Receptor 4 expressed in E. coli.
Recognizes human Toll-like Receptor 4 (TLR4).
Immunohistochemistry: LS-B2254 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2254 was determined to be 5 ug/ml.Flow Cytometry: 3ug/10e6 cellsOptimal dilutions to be determined by researcher.

Packaging

Borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About TLR4

O00206 NM_138554 O00206

TLR4 Antibody, CD284 Antibody, CD284 antigen Antibody, Homolog of Drosophila toll Antibody, TLR-4 Antibody, TOLL Antibody, ARMD10 Antibody, Toll-like receptor 4 Antibody, HToll Antibody

Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni2+. Responses triggered by Ni2+ require non-conserved histidines and are, therefore, species-specific.

Immunohistochemistry

Immunohistochemistry
Anti-TLR4 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2254 concentration 5 ug/ml.
 

Requested From: United States
Date Requested: 12/5/2016

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