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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2254-50 | 50 µg (0.5 mg/ml) | $485 |
IHC‑plus™ Monoclonal Mouse anti‑Human TLR4 Antibody (clone 1E11, IHC) LS‑B2254
IHC‑plus™ Monoclonal Mouse anti‑Human TLR4 Antibody (clone 1E11, IHC) LS‑B2254
Note: This antibody replaces LS-C10724, LS-C149672
Antibody:
TLR4 Mouse anti-Human Monoclonal (1E11) Antibody
Application:
IHC, IHC-P, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
TLR4 Mouse anti-Human Monoclonal (1E11) Antibody
Application:
IHC, IHC-P, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
TLR4 antibody LS-B2254 is an unconjugated mouse monoclonal antibody to human TLR4. Validated for ELISA, Flow and IHC. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human TLR4
Synonyms
TLR4 | CD284 | CD284 antigen | Homolog of Drosophila toll | TLR-4 | TOLL | ARMD10 | Toll-like receptor 4 | HToll
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Clone
1E11
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Immunogen
Recombinant protein corresponding to amino acids 238-609 of human Toll-like Receptor 4 expressed in E. coli.
Specificity
Recognizes human Toll-like Receptor 4 (TLR4).
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Flow Cytometry
- ELISA (1:2000 - 1:4000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2254 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2254 was determined to be 5 ug/ml.Flow Cytometry: 3ug/10e6 cellsOptimal dilutions to be determined by researcher.
Presentation
Borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Validation
Anti-TLR4 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TLR4 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ TLR4 Antibody (clone 1E11) for IHC, Flow, ELISA LS-B2254 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Age-associated skin changes in innate immunity markers reflect a complex interaction between aging mechanisms in the sebaceous gland. Rana M Elewa, Marwa A Abdallah, Christos C Zouboulis. The Journal of dermatology. 2015 May;42:467-76.
Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Species:
Human, Guinea pig
Applications:
IHC, IHC - Frozen, Immunofluorescence, Flow Cytometry
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, ICC, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
