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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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TLR4 Antibody (C‑Terminus) LS‑C116816
TLR4 antibody LS-C116816 is an unconjugated rabbit polyclonal antibody to human TLR4. Validated for WB.
Catalog
Size
Price
LS-C116816-50
50 µg
Unavailable

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Product Description

TLR4 antibody LS-C116816 is an unconjugated rabbit polyclonal antibody to human TLR4. Validated for WB.
About TLR4
Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni2+. Responses triggered by Ni2+ require non-conserved histidines and are, therefore, species-specific. O00206 NM_138554 O00206

TLR4 Antibody, CD284 Antibody, CD284 antigen Antibody, Homolog of Drosophila toll Antibody, TLR-4 Antibody, TOLL Antibody, ARMD10 Antibody, Toll-like receptor 4 Antibody, HToll Antibody


Specifications

Target
Human TLR4
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
TLR4 antibody was raised against synthetic peptide from C-Terminus of human TLR4 (O00206, NP_612564). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon (100%).
Blocking Peptide
LS-E21635 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C116816. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human TLR4
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

TLR4 antibody LS-C116816 Western blot of A549 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TLR4 antibody LS-C116816 Western blot of A549 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

TLR4 antibody LS-C116816 Western blot of A549 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TLR4 antibody LS-C116816 Western blot of A549 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

TLR4 antibody LS-C116816 Western blot of A549 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TLR4 antibody LS-C116816 Western blot of A549 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

TLR4 antibody LS-C116816 Western blot of A549 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TLR4 antibody LS-C116816 Western blot of A549 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/19/2018

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