TLR4 antibody LS-C10716 is a biotin-conjugated mouse monoclonal antibody to human TLR4. Validated for ELISA, Flow, Func and IP.
(tested or 100% immunogen sequence identity)
- Flow Cytometry
- ELISA (1 µg/ml)
- Functional Assay
TLR4 antibody was raised against ba/F3 cell line expressing human TLR4 cell surface antigen.
Recognizes human TLR4 (Toll Like Receptor 4) (Biotin).
ELISA, Flow cytometry. Functional studies: It has been shown to block the activation of monocytes with LPS). Immunoprecipitation: suitable. Recommended Dilution: ELISA: 1 ug/ml. Flow cytometry: 2-5 ug/1 x 10 cells. However, the optimal condition has to be determined for individual experiments. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, 0.02% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.