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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C367232-100 100 µg (1 mg/ml) $365 
TLR4 Antibody - Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [Azide Free] - Formalin-fixed, paraffin-embedded human testis tissue stained with TLR4 antibody (5 ug/ml). This image was taken for the unmodified form of this product. Other forms have not been tested.
TLR4 Antibody - Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [Azide Free] - Human skin stained with TLR4 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. This image was taken for the unmodified form of this product. Other forms have not been tested.
TLR4 Antibody - Western Blot: TLR4 Antibody (76B357.1) [Azide Free] - Analysis of TLR4 using TLR4 antibody at 2 ug/ml on (A) human intestine and 6 ug/ml on (B) mouse intestine and C) rat intestine lysate. This image was taken for the unmodified form of this product. Other forms have not been tested.
TLR4 Antibody - Flow Cytometry: TLR4 Antibody (76B357.1) [Azide Free] - Analysis of cell surface TLR4 in mouse splenocytes using 0.5 ug/10^6 cells of TLR4 antibody. Shaded histogram represents cells without TLR4 antibody; green represents isotype control (BD Pharmingen) red represents TLR4 antibody. This image was taken for the unmodified form of this product. Other forms have not been tested.
TLR4 Antibody - Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [Azide Free] - Formalin-fixed, paraffin-embedded human testis tissue stained with TLR4 antibody (5 ug/ml). This image was taken for the unmodified form of this product. Other forms have not been tested.
TLR4 Antibody - Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [Azide Free] - Human skin stained with TLR4 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. This image was taken for the unmodified form of this product. Other forms have not been tested.
TLR4 Antibody - Western Blot: TLR4 Antibody (76B357.1) [Azide Free] - Analysis of TLR4 using TLR4 antibody at 2 ug/ml on (A) human intestine and 6 ug/ml on (B) mouse intestine and C) rat intestine lysate. This image was taken for the unmodified form of this product. Other forms have not been tested.
TLR4 Antibody - Flow Cytometry: TLR4 Antibody (76B357.1) [Azide Free] - Analysis of cell surface TLR4 in mouse splenocytes using 0.5 ug/10^6 cells of TLR4 antibody. Shaded histogram represents cells without TLR4 antibody; green represents isotype control (BD Pharmingen) red represents TLR4 antibody. This image was taken for the unmodified form of this product. Other forms have not been tested.
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TLR4 Antibody (aa100‑200, clone 76B357.1, Azide‑free) LS‑C367232

TLR4 Antibody (aa100‑200, clone 76B357.1, Azide‑free) LS‑C367232

Antibody:
Mouse Monoclonal to Human TLR4
Application:
IHC, IHC-P, ICC, IF, WB, Flo
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Azide-free
Other formats:
Price
Catalog Number
$365 
LS-C367232-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human TLR4
Application:
IHC, IHC-P, ICC, IF, WB, Flo
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Azide-free
Other formats:

Specifications

Description
TLR4 antibody LS-C367232 is an unconjugated mouse monoclonal antibody to TLR4 from human, mouse and rat. Validated for Flow, ICC, IF, IHC and WB.
Target
Human TLR4
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG2b,k Monoclonal
Clone
76B357.1
Conjugations
Unconjugated. Also available conjugated with AF488.
Purification
Protein G purified
Modifications
Azide-free
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ICC (1:10 - 1:500)
  • Immunofluorescence (1:10 - 1:500)
  • Western blot (1 - 3 µg/ml)
  • Flow Cytometry (0.5 - 2 µg/10E6 cells)
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
This antibody was developed against a portion of amino acids 100-200 of human TLR4 (NP_612564).
Epitope
aa100-200
Specificity
Human TLR4. The amino acid sequence used has 100% identity in human, chimp, baboon, Chinese hamster, and rat; 93% identity in bovine and pig; 85% identity in sheep and 78% identity in cat, horse and mouse.
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Presentation
PBS
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TLR4
O00206 NM_138554 O00206

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Requested From: United States
Date Requested: 12/14/2019