Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [LD5]
Specificity and Use
TLR1 antibody was raised against mouse TLR1
This LD5 antibody has been tested by immunoblotting of mouse TLR1-transfected, peritoneal-exudate, and RAW264.7 cells. This can be used at 1-3 ug/ml for western blotting analysis. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PBS, pH 7.2, 150 mM sodium chloride, 0.09% sodium azide
TLR1 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity.
Left Panel: Untransfected control (left) and mouse TLR1-transfected (right) Baf/3 cell lysates were loaded at 1x105 cells/lane, probed with 3 ug/mL purified antibody and revealed with HRP anti-rat IgG. Right Panel: Peritoneal-exudate (left) and RAW264.7 (right) cell lysates were loaded at 1x105 cells/lane, probed with 3 ug/ml purified antibody and revealed with HRP anti-rat IgG.
Requested From: United States
Date Requested: 10/23/2016