(tested or 100% immunogen sequence identity)
- IHC - Frozen (1:200 - 1:1000)
- Western blot
- ELISA (1:200 - 1:1000)
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- IHC - 4% paraformaldehyde
TLE2 antibody was raised against synthetic peptide from human Groucho 2 (aa 220-235, KEPSGPYESDEDKSDY) conjugated to BSA. Percent identity by BLAST analysis: Human (100%); Gorilla, Gibbon (94%); Elephant, Dog, Bovine, Horse (88%); Marmoset (81%).
Recognizes Groucho 2 / TLE2 / ESG2 transcription factor. Species cross-reactivity: Human and rodent.
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Immunohistochemistry (Frozen sections): 1:200-1:1000. Not tested for paraffin. Western Blot: 1:200-1:1000. ELISA: 1:200-1:1000.
PBS, pH 7.2, No preservatives added
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.