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Anti-TJP1 / ZO-1 Antibody LS-C18590

Catalog Size Price
LS-C18590-100 100 µg (0.25 mg/ml) Unavailable

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63 TJP1 / ZO-1 Antibodies

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Anti-TJP1 / ZO-1 Antibody (aa1-296) IHC-plus™ LS-B11010
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100% Guaranteed
Rabbit Polyclonal (IgG) to Human TJP1 / ZO-1
Human
IHC - Frozen, Immunofluorescence, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human TJP1 / ZO-1
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, MaxLight 405, MaxLight 490, MaxLight 550, MaxLight 650, MaxLight 750, PE.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Frozen
  • Immunofluorescence (2.5 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

TJP1 / ZO-1 antibody was raised against synthetic peptide derived from the mid region of human ZO-1, which differs from dog, mouse, and rat by one conservative amino acid replacement.
Recognizes human ~225kD ZO-1 protein. Bands of unknown origin may be observed at lower molecular weights in some sample lysates. These bands may represent breakdown products. Reactivity has been confirmed with dog MDCK-II, human A431 and Caco-2, rat Rat-1 and NRK-52E cell lysates by Western Blot and with MDCK-II, Caco-2, Rat-1 and NRK-52E cells by Immunofluorescence. Reactivity has also been confirmed with frozen mouse liver, blood vessel (in heart) and mossy fiber terminal (in brain) tissues by Immunohistochemistry and Immunofluorescence.
Suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry. Western Blot: 1-3 ug/ml. Immunofluorescence: 2.5 ug/ml. Immunohistochemistry (frozen): 2 ug/ml.

Packaging

PBS, pH 7.4, 0.1% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About TJP1 / ZO-1

Q07157 NM_003257 NP_003248.3

TJP1 Antibody, ZO-1 Antibody, Zona occludens protein 1 Antibody, Zona occludens 1 Antibody, Zonula occludens protein 1 Antibody, Tight junction protein 1 Antibody, ZO1 Antibody, Tight junction protein ZO-1 Antibody, Zonula occludens 1 protein Antibody

The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.

Requested From: 
Date Requested: 6/28/2017

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