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Anti-TJP1 / ZO-1 Antibody (aa463-1109) IHC-plus™ LS-B2129

Note: This antibody replaces LS-C18588

Ordering

Wt. Vol. Conc. Price
50 µg - 0.25 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular TJP1 / ZO-1 Antibodies

Anti-TJP1 / ZO-1 Antibody (C-Terminus) IHC-plus™ LS-B9774
Goat Polyclonal (IgG) to Human TJP1 / ZO-1
Human, Monkey, Mouse, Rat, Dog
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human TJP1 / ZO-1
Human, Mouse, Rat, Dog, Guinea pig
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human TJP1 / ZO-1
Rabbit
Human, Mouse, Rat, Dog, Guinea pig (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • IHC - Frozen
  • Western blot (0.5 - 2 µg/ml)
  • Immunoprecipitation
  • ELISA

Specificity and Use

TJP1 / ZO-1 antibody was raised against a 69kD fusion protein corresponding to aa463-1109 of human ZO-1 cDNA. This sequence lies N-terminal to the 80 amino acid region (the alpha-motif) present in the a+-isoform but absent in the a- isoform due to alternative splicing.
aa463-1109
Recognizes human ZO-1alpha+ and ZO- alpha- isoforms. Reactivity with dlg has not been characterized. No reactivity with PSD-95 or p55 has been observed on Western blots, but potential reactivity with these proteins has not been fully characterized. Predicted species cross-reactivity: mouse, rat, guinea pig, canine and chicken (moderate reactivity). Please note that cross-reactivity with predicted species is only predicted and is not guaranteed.
Immunohistochemistry: LS-B2129 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2129 was determined to be 5 ug/ml. Western Blot: 0.5-2 ug/ml. Immunoprecipitation: 2-5 ug/reaction.

Packaging

PBS, pH 7.4, 0.09% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About TJP1 / ZO-1

Q07157 NM_003257 NP_003248.3

TJP1 Antibody, ZO-1 Antibody, Zona occludens protein 1 Antibody, Zona occludens 1 Antibody, Zonula occludens protein 1 Antibody, Tight junction protein 1 Antibody, ZO1 Antibody, Tight junction protein ZO-1 Antibody, Zonula occludens 1 protein Antibody

The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.

Immunohistochemistry

Immunohistochemistry
Anti-ZO-1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2129 concentration 5 ug/ml.
 

Requested From: United States
Date Requested: 12/10/2016

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