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Anti-TIP120 / CAND1 Antibody (N-Terminus) IHC-plus™ LS-B347

Note: This antibody replaces LS-C18987

Ordering

Wt. Vol. Conc. Price
- 50 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular TIP120 / CAND1 Antibodies

Anti-TIP120 / CAND1 Antibody (aa112-161) LS-C31482
Rabbit Polyclonal (IgG) to Human TIP120 / CAND1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Western blot
Unconjugated
Western blot Image
Anti-TIP120 / CAND1 Antibody (N-Terminus) IHC-plus™ LS-B347
Rabbit Polyclonal to Human TIP120 / CAND1
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-TIP120 / CAND1 Antibody (aa11-24) LS-C127211
Rabbit Polyclonal to Human TIP120 / CAND1
Human, Monkey, Mouse, Rat, Bat, Bovine, Pig, Xenopus, Zebrafish
Western blot, Immunoprecipitation, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed Publications Publications (3)
Rabbit Polyclonal to Human TIP120 / CAND1
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human TIP120 / CAND1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Delipidated and defibrinated
Unmodified

Applications

  • IHC - Paraffin (1:500)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation
  • ELISA (1:2000 - 1:10000)

Specificity and Use

TIP120 / CAND1 antibody was raised against synthetic peptide from human CAND1.
N-Terminus
Amino acids 11-24 of Human CAND1/TIP120A (N-terminal) coupled to KLH.
Immunohistochemistry: LS-B347 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B347 was determined to be 1:500.

Packaging

Serum - liquid, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

Publications (3)

1
NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases. Liu J, Furukawa M, Matsumoto T, Xiong Y. Molecular cell. 2002 10:1511-8. [PubMed:12504025]
2
AtCAND1, a HEAT-repeat protein that participates in auxin signaling in Arabidopsis. Cheng Y, Dai X, Zhao Y. Plant physiology. 2004 135:1020-6. [PubMed:15181201] [PMC:PMC514136]
3
Arabidopsis CAND1, an unmodified CUL1-interacting protein, is involved in multiple developmental pathways controlled by ubiquitin/proteasome-mediated protein Degradation. Feng S, Shen Y, Sullivan JA, Rubio V, Xiong Y, Sun TP, Deng XW. The Plant cell. 2004 16:1870-82. [PubMed:15208391] [PMC:PMC514167]

About TIP120 / CAND1

Q86VP6 NM_018448 NP_060918.2

CAND1 Antibody, KIAA0829 Antibody, TBP interacting protein Antibody, TBP-interacting protein 120A Antibody, TIP120 Antibody, p120 CAND1 Antibody, TIP120A Antibody

Key assembly factor of SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes that promotes the exchange of the substrate-recognition F-box subunit in SCF complexes, thereby playing a key role in the cellular repertoire of SCF complexes. Acts as a F-box protein exchange factor.

Immunohistochemistry

Immunohistochemistry
Anti-CAND1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B347 dilution 1:500.
 

Requested From: United States
Date Requested: 12/7/2016

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