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Anti-TIMP1 Antibody IHC-plus™ LS-B7485

Note: This antibody replaces LS-C104830

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular TIMP1 Antibodies

Anti-TIMP1 Antibody LS-C39715
Rabbit Polyclonal (IgG) to Human TIMP1
Human, Rat, Bovine, Ferret, Horse, Rabbit, Sheep
IHC - 4% paraformaldehyde, Western blot, ELISA
Unconjugated
Anti-TIMP1 Antibody (aa25-205) LS-C150260
Goat Polyclonal (IgG) to Mouse TIMP1
Mouse
IHC, Western blot, Immunoprecipitation, Neutralization
Unconjugated
Immunohistochemistry Image
Anti-TIMP1 Antibody (Biotin) LS-C317517
Rabbit Polyclonal (IgG) to Human TIMP1
Human
IHC, IHC - Paraffin, Western blot, ELISA
Biotin Conjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human TIMP1
Human
IHC - Paraffin, Western blot, ELISA, Neutralization
Unconjugated

Details

Human TIMP1
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
  • Neutralization (5 µg/ml)

Specificity and Use

TIMP1 antibody was raised against e.coli derived recombinant Human TIMP-1.
Human TIMP-1
Immunohistochemistry: LS-B7485 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human TIMP-1 (3 ug/ml), a concentration of 5 ug/ml of this antibody is required. ELISA: To detect hTIMP-1 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TIMP-1 (LS-C104724) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTIMP-1. Western Blot: To detect hTIMP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTIMP-1 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
50 µl Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About TIMP1

P01033 NM_003254 NP_003245.1

TIMP1 Antibody, CLGI Antibody, EPA Antibody, HCI Antibody, Metalloproteinase inhibitor 1 Antibody, TIMP-1 Antibody, Collagenase inhibitor Antibody

Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1.

Immunohistochemistry

Immunohistochemistry
Anti-TIMP1 antibody IHC of human prostate, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7485 concentration 5 ug/ml.

Western blot

Western blot
Western Blot (reducing) of TIMP-1 antibody LS-B7485

Western blot

Western blot
Western Blot (non-reducing) of TIMP-1 antibody LS-B7485

Western blot

Western blot
Western Blot (reducing) of TIMP-1 antibody LS-B7485

Western blot

Western blot
Western Blot (non-reducing) of TIMP-1 antibody LS-B7485

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of TIMP-1 antibody LS-B7485

Requested From: United States
Date Requested: 12/8/2016

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