Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat Monoclonal [clone FF-10] (IgG1) to Mouse Thy 1.2
IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Mouse Thy 1.2
Mouse (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [FF-10]
Protein G purified
IHC - Frozen
Flow Cytometry (1:50 - 1:100)
Specificity and Use
Thy 1.2 antibody was raised against eL-4 cells.
Recognizes the mouse Thy1.2 cell surface antigen and binds to T cell lines (EL-4, 3A9), T lymphocytes and the WEH1-3 cell line. 3T3 fibroblasts are also stained by this antibody. This clone recognizes a 30kD glycoprotein in immunoprecipitation and Western blotting assays. The antibody strongly induces homotypic adhesion of Thy1.2 positive cell lines.
Flow Cytometry:1:50-1:100. Immunohistochemistry (frozen). Immunoprecipitation. Western Blot.
PBS, pH 7.2, 0.1% sodium azide
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months.