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Anti-Thrombospondin 1 Antibody (clone A6.1, Azide-free) IHC-plus™ LS-B2570

Note: This antibody replaces LS-C26351, LS-C87486

Ordering

Wt. Vol. Conc. Price
- 50 µl 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Thrombospondin 1 Antibodies

Anti-Thrombospondin 1 Antibody (Azide-free) IHC-plus™ LS-B2156
Mouse Monoclonal (IgG1) to Human Thrombospondin 1
Human
IHC - Paraffin, Western blot
Unconjugated, Azide-free
Immunohistochemistry Image
Anti-Thrombospondin 1 Antibody (clone A6.1, Azide-free) IHC-plus™ LS-B2570
Mouse Monoclonal [clone A6.1] (IgG1) to Human Thrombospondin 1
Human, Mouse, Rat, Bovine, Dog, Horse, Pig, Sheep
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated, Azide-free
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone A6.1] (IgG1) to Human Thrombospondin 1
Human, Mouse, Rat, Bovine, Dog, Horse, Pig, Sheep
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated, Azide-free

Details

Human Thrombospondin 1
Mouse
Human, Mouse, Rat, Bovine, Dog, Horse, Pig, Sheep (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [A6.1]
Unconjugated
Protein G purified
Azide-free

Applications

  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry

Specificity and Use

Reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets. MW of Antigen: ~450kD (non-reduced), 170-180kD (reduced). Epitope: Collagen Type V-binding domain of TSP.
Recognizes Thrombospondin. Shows no cross-reactivity with fibronectin, fibrinogen and von Willebrand factor. Shows a mild cross-reaction with TSP 2. Inhibits TSP-collagen interaction. Antibody binding to TSP is unaffected by glycosaminoglycans (e. g. hyaluronic acid, chondroitin sulfate and heparin). Its binding is enhanced by EDTA i. e. at low concentration of Ca2+. Species cross-reactivity: Human, bovine, porcine, equine, canine, sheep, mouse and rat.
Immunohistochemistry: LS-B2570 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2570 was determined to be 10 ug/ml. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Packaging

PBS, pH 7.4.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About Thrombospondin 1


Immunohistochemistry

Immunohistochemistry
Anti-Thrombospondin antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2570 concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/7/2016

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