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Catalog Number Size Price
LS-C112966-100 100 µg (0.5 mg/ml) $503 
THEMIS / SPOT Antibody - THEMIS antibody (0.3 ug/ml) staining of Mouse Spleen lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Polyclonal Goat anti‑Mouse THEMIS / SPOT Antibody (aa577‑590, WB) LS‑C112966

Polyclonal Goat anti‑Mouse THEMIS / SPOT Antibody (aa577‑590, WB) LS‑C112966

Antibody:
THEMIS / SPOT Goat anti-Mouse Polyclonal (aa577-590) Antibody
Application:
WB, Peptide-ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$503
LS-C112966-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
THEMIS / SPOT Goat anti-Mouse Polyclonal (aa577-590) Antibody
Application:
WB, Peptide-ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
SPOT antibody LS-C112966 is an unconjugated goat polyclonal antibody to mouse SPOT (THEMIS) (aa577-590). Validated for Peptide-ELISA and WB.
Target
Mouse THEMIS / SPOT
Synonyms
THEMIS | C6orf207 | C6orf190 | BA325O24.3 | GRB2-associated protein | Protein THEMIS | SPOT | Thymocyte selection associated | BA325O24.4 | TSEPA | GASP
Host
Goat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-EERTINLPKSLKSH, from the C-Terminus of protein sequence according to NP_848781.1.
Epitope
aa577-590
Specificity
Mouse THEMIS.
Applications
  • Western blot (1 - 3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 72kDa band observed in Mouse Spleen lysates (calculated MW of 72.7kDa according to NP_848781.1). Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About THEMIS / SPOT
Q8BGW0 NP_848781.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024