LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-THEMIS / SPOT Antibody (aa577-590) LS-C112966

Ordering

Wt. Vol. Conc. Price
100 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular THEMIS / SPOT Antibodies

Anti-THEMIS / SPOT Antibody (aa559-588, AP) LS-C241770
Rabbit Polyclonal (IgG) to Human THEMIS / SPOT
Human, Mouse
IHC, Western blot, ELISA
AP Conjugated
Anti-THEMIS / SPOT Antibody (aa559-588, PE) LS-C241776
Rabbit Polyclonal (IgG) to Human THEMIS / SPOT
Human, Mouse
IHC, Western blot, ELISA
PE Conjugated
Anti-THEMIS / SPOT Antibody (aa559-588, Azide-free, HRP) LS-C247062
Rabbit Polyclonal (IgG) to Human THEMIS / SPOT
Human, Mouse
IHC, Western blot, ELISA
HRP Conjugated, Azide-free

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Mouse THEMIS / SPOT
Mouse
Western blot, ELISA
Unconjugated

Details

Mouse THEMIS / SPOT
Goat
Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1 - 3 µg/ml)
  • ELISA (1:64000)

Specificity and Use

THEMIS / SPOT antibody was raised against synthetic peptide C-EERTINLPKSLKSH from the C-terminus of mouse THEMIS (NP_848781.1). Percent identity with other species by BLAST analysis: Mouse (100%).
LS-E27328 - Lyophilized - 100 µg - $145.00
aa577-590
Mouse THEMIS.
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 72kDa band observed in Mouse Spleen lysates (calculated MW of 72.7kDa according to NP_848781.1). Recommended concentration: 1-3 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About THEMIS / SPOT

Q8BGW0 NP_848781.1

THEMIS Antibody, C6orf207 Antibody, C6orf190 Antibody, BA325O24.3 Antibody, GRB2-associated protein Antibody, Protein THEMIS Antibody, SPOT Antibody, Thymocyte selection associated Antibody, BA325O24.4 Antibody, TSEPA Antibody, GASP Antibody

Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk.

Western blot

Western blot
THEMIS antibody (0.3 ug/ml) staining of Mouse Spleen lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
 

Requested From: United States
Date Requested: 12/4/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number