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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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THBS3 / Thrombospondin 3 Antibody (aa936‑950) IHC‑plus™ LS‑B9501 Best Seller
Note: This antibody replaces LS-C112816
Thrombospondin 3 antibody LS-B9501 is an unconjugated goat polyclonal antibody to Thrombospondin 3 (THBS3) from human, mouse, rat and other species. Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B9501-50
50 µg
Unavailable

Popular THBS3 / Thrombospondin 3 Products

Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Mouse
Applications: Western blot, ELISA
Western blot of THBS3 / Thrombospondin 3 antibody.
Species: Human
Applications: IHC, Western blot
Immunohistochemistry of paraffin-embedded human epityphlon using antibody at 1:100 dilution.
Species: Human
Applications: IHC, IHC - Paraffin, ELISA

Product Description

Thrombospondin 3 antibody LS-B9501 is an unconjugated goat polyclonal antibody to Thrombospondin 3 (THBS3) from human, mouse, rat and other species. Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
About THBS3 / Thrombospondin 3
THBS3 / Thrombospondin 3 belongs to the thrombospondin family. Thrombospondin family members are adhesive glycoproteins that mediate cell-to-cell and cell-to-matrix interactions. This protein forms a pentameric molecule linked by a single disulfide bond. This gene shares a common promoter with metaxin 1. Alternate splicing results in coding and non-coding transcript variants. P49746 NM_007112 NP_009043.1

THBS3 Antibody, Thrombospondin-3 Antibody, Thrombospondin 3 Antibody, TSP3 Antibody


Specifications

Target
Human THBS3 / Thrombospondin 3
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide CNDTVPEDFEPFRRQ from the C-terminus of human THBS3 (NP_009043.1). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig (100%); Xenopus, Zebrafish (80%).
Blocking Peptide
LS-E26998 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B9501. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa936-950
Specificity
Human THBS3.
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Preliminary experiments gave an approx 50kDa band in Human Lung and in lysates of cell line A549 after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 104kDa according to NP_009043.1. The 50kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P with formalin fixed tissues and HIER antigen retrieval. The antibody does not work in IHC using samples fixed in 4% PFA.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunohistochemistry

Anti-THBS3 / Thrombospondin 3 antibody IHC staining of human vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-THBS3 / Thrombospondin 3 antibody IHC staining of human vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-THBS3 / Thrombospondin 3 antibody IHC staining of human vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-THBS3 / Thrombospondin 3 antibody IHC staining of human vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-THBS3 / Thrombospondin 3 antibody IHC staining of human vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-THBS3 / Thrombospondin 3 antibody IHC staining of human vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-THBS3 / Thrombospondin 3 antibody IHC staining of human vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-THBS3 / Thrombospondin 3 antibody IHC staining of human vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.


Requested From: 
Date Requested: 6/18/2018

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