Rat TH / Tyrosine Hydroxylase

Sheep

Rat (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

Native tyrosine hydroxylase from rat pheochromocytoma.

Recognizes tyrosine hydroxylase. Cross-reacts with all mammalian forms and some non-mammalian forms.

• IHC (0.02 - 0.1 µg/ml)
• Western blot (0.05 - 0.1 µg/ml)

Suitable for use in Western Blot and Immunohistochemistry (peroxidase). Western Blot: 0.05-0.1 ug/ml readily detected 1-10 ng of TH/lane using 125I-protein A. Immunohistochemistry: 0.02-0.1 ug/ml produced specific labeling of noradrenergic axons in primate cerebral cortex. Using horseradish peroxidase (HRP). Note: It is important to use a secondary antibody that has significant reactivity to sheep IgG1 since the majority of the sheep immunoglobulin is IgG1.

Dulbeccos PBS, 10% glycerol, 10 mM HEPES, 20 mM Tris-HCl, 0.1% PVP-40, 0.05% Tween-20, 0.1% sodium azide, 1 mg/ml BSA.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P07101 NCBI: NM_000360 NP_000351.2